文献类型：期刊文献

英文题名：Programming Integrative Multienzyme Systems and Ionic Strength for Recyclable Synthesis of Glutathione

作者：Cui, Xiangwei[1]; Wang, Zeyuan[1]; Li, Zonglin[1]; Zhang, Xing[1]; Li, Zhimin[1,2]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China; [2] Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai, 200237, China

年份：2021

卷号：69

期号：13

起止页码：3887

外文期刊名：Journal of Agricultural and Food Chemistry

收录：EI(收录号：20211810292284)

语种：英文

外文关键词：Ionic strength - Peptides - Genes - Enzymes - Catalysis

摘要：In the enzymatic cascade catalysis, it is a big challenge to construct a stable and reusable catalyst with targeted enzymes. The artificial multienzyme reactor has attracted great attention due to its potential for facilitating the performance of enzyme catalysis. In this study, we set up a reliable system that could assemble polyphosphate kinase (PPK) with bifunctional glutathione synthetase (GshF) via SpyCatcher/SpyTag to form multienzyme systems (MESs). Furthermore, MESs could assemble into nanoaggregates by altering the ionic strength, and the larger nanoaggregates could be applied in robust and reusable synthesis of glutathione (GSH). To enhance MES levels in vivo, gene duplication and different coexpression modes were performed. Finally, the optimized production of GSH and oxidized glutathione (GSSG) reached 102.6 and 6.7 mM within 2 h. Compared with the first round, the total yield only decreased by 9.4% after five continuous rounds of biocatalysis. ?