文献类型：期刊文献

英文题名：EFFECTS OF PRECURSORS ON KITASAMYCIN PRODUCTION IN Streptomyces kitasatoensis

作者：Zheng, Xiong[1];Ye, Ruifang[1];Ho, Fengxian[1];Mao, Quangui[2];Zhang, Hongzhou[2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Topfond Pharmaceut Co Ltd, Zhengzhou, Henan, Peoples R China

年份：2019

卷号：35

期号：3

起止页码：910

外文期刊名：BIOSCIENCE JOURNAL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000477739200027)】；

基金：This work was supported by Topfond Pharmaceutical Co., Ltd., Henan, China.

语种：英文

外文关键词：Streptomyces kitasatoensis; Ethylacetate; Fermentation; Component; Kitasamycin

摘要：To improve kitasamycin biosynthesis by Streptomyces kitasatoensis Z-7, the addition of two precursors, sodium acetate and ethyl acetate, to the fermentation medium was evaluated. Ethyl acetate was the most effective precursor compared with control conditions; In a 15-L fermentor, the kitasamycin titer was 21% higher when 0.48% ethyl acetate was added compared to control conditions. Content of the A(5) component increased by 5.1%, and the A(4) content decreased slightly compared to that of the control. During kitasamycin synthesis, intracellular and extracellular concentrations of acetic acid were higher for S. kitasatoensis Z-7 supplemented with ethyl acetate than for the non-supplemented strain, and the activities of acyl-CoA synthetases, acyl-phosphotransferases, and acyl-kinases were also significantly increased, suggesting that increased acetyl-CoA levels can explain the high kitasamycin titer. These findings may improve the industrial-scale production of kitasamycin for clinical use, and the addition of 0.48% ethyl acetate as precursors in the medium at the beginning of cultivation was a new method to mitigate the negative influence on the cell growth of excess precursor.