文献类型：期刊文献

英文题名：Metabolic capability and in situ activity of microorganisms in an oil reservoir

作者：Liu, Yi-Fan[1,2,3];Galzerani, Daniela Domingos[3];Mbadinga, Serge Maurice[1,2,5];Zaramela, Livia S.[3];Gu, Ji-Dong[4];Mu, Bo-Zhong[1,2,5];Zengler, Karsten[3,6]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Univ Calif San Diego, Dept Pediat, 9500 Gilman Dr, San Diego, CA 92093 USA;[4]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China;[6]Univ Calif San Diego, Ctr Microbiome Innovat, 9500 Gilman Dr, San Diego, CA 92093 USA

年份：2018

卷号：6

外文期刊名：MICROBIOME

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000419616900001)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41530318, 41373070) to YFL, SMM, and BZM and the NSFC/RGC Joint Research Fund (No. 41161160560) to JDG. China Scholarship Council (CSC) funded Yi-Fan Liu's doctoral scholarship in a joint-PhD program with the University of California, San Diego. Additionally, Daniela Domingos Galzerani was supported by CNPq under the Brazilian Scientific Mobility Program, Ciencias sem Fronteiras (No 201011/2014-0). This study is in part based on work supported by the US Department of Energy (DOE), Office of Science, Office of Biological & Environmental Research Awards DE-SC0012586 and the Frontiers of Innovation Scholars Program at UCSD.

语种：英文

外文关键词：Auxotrophy; Hydrocarbon degradation; Metagenomics and metatranscriptomics; Microbial community; Oil reservoir

摘要：Background: Microorganisms have long been associated with oxic and anoxic degradation of hydrocarbons in oil reservoirs and oil production facilities. While we can readily determine the abundance of microorganisms in the reservoir and study their activity in the laboratory, it has been challenging to resolve what microbes are actively participating in crude oil degradation in situ and to gain insight into what metabolic pathways they deploy. Results: Here, we describe the metabolic potential and in situ activity of microbial communities obtained from the Jiangsu Oil Reservoir (China) by an integrated metagenomics and metatranscriptomics approach. Almost complete genome sequences obtained by differential binning highlight the distinct capability of different community members to degrade hydrocarbons under oxic or anoxic condition. Transcriptomic data delineate active members of the community and give insights that Acinetobacter species completely oxidize alkanes into carbon dioxide with the involvement of oxygen, and Archaeoglobus species mainly ferment alkanes to generate acetate which could be consumed by Methanosaeta species. Furthermore, nutritional requirements based on amino acid and vitamin auxotrophies suggest a complex network of interactions and dependencies among active community members that go beyond classical syntrophic exchanges; this network defines community composition and microbial ecology in oil reservoirs undergoing secondary recovery. Conclusion: Our data expand current knowledge of the metabolic potential and role in hydrocarbon metabolism of individual members of thermophilic microbial communities from an oil reservoir. The study also reveals potential metabolic exchanges based on vitamin and amino acid auxotrophies indicating the presence of complex network of interactions between microbial taxa within the community.