文献类型：期刊文献

英文题名：Epidemiological distribution of genotypes of Giardia duodenalis in humans in Spain

作者：Wang, Yuanfei[1];Gonzalez-Moreno, Olga[2,3];Roellig, Dawn M.[4];Oliver, Laura[2];Huguet, Jordi[2];Guo, Yaqiong[5];Feng, Yaoyu[1,5];Xiao, Lihua[5]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]SYNLAB, Lab Microbiol & Parasitol, Barcelona 08950, Spain;[3]Univ Barcelona, Fac Pharm, Dept Biol Healthcare & Environm, Lab Parasitol, E-08028 Barcelona, Spain;[4]Ctr Dis Control & Prevent, Div Foodborne Waterborne & Environm Dis, Atlanta, GA 30333 USA;[5]South China Agr Univ, Coll Vet Med, Key Lab Zoonosis, Minist Agr, Guangzhou 510642, Guangdong, Peoples R China

年份：2019

卷号：12

期号：1

外文期刊名：PARASITES & VECTORS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000484916800003)】；

基金：This work was supported by the National Key R&D Programme of China (2017YFD0501305 to YYF). YFW was partially supported by the China Scholarship Council. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Giardia duodenalis; Multilocus genotyping; Assemblage; Spain

摘要：Background Although the distribution of Giardia duodenalis genotypes in humans has been increasingly reported in recent years, data on possible differences in pathogen transmission between age groups and virulence between genotypes are scarce. The purpose of this study is to investigate the genetic diversity of G. duodenalis in humans in Spain and compare the distribution of G. duodenalis assemblages A and B between children and adults and clinical presentations between the two genotypes. Methods In the present study, 125 microscopy-positive fecal samples were collected from humans in Spain over a 7-year period. PCR and sequence analyses of the triosephosphate isomerase, beta-giardin and glutamate dehydrogenase genes were used to identify the multilocus genotypes of G. duodenalis. Results Sequence analysis of three genetic loci identified both G. duodenalis assemblages A (29) and B (66), with co-infections of the two in two patients. Among the sequences obtained in this study, four multilocus genotypes (MLGs) of the sub-assemblage AII were observed within assemblage A. In contrast, 19 MLGs were detected within assemblage B due to the high sequence diversity at each locus. One MLG, however, was found in 51.9% (27/52) of assemblage B samples. Children were more commonly infected by assemblage B (44/53 or 83%) than adults (22/42 or 52.4%; chi(2) = 10.371, df = 1, P = 0.001). Asymptomatic infection was more common in patients with assemblage A (4/29 or 13.8%) than in those with assemblage B (1/66 or 1.5%; chi(2) = 6.091, df = 1, P = 0.029), and the frequency of abdominal pain occurrence was higher in assemblage B patients (65/66 or 98.5%) than assemblage A patients (25/29 or 86.2%; chi(2) = 6.091, df = 1, P = 0.029). Conclusions These results illustrate the existence of differences in genotype distribution between children and adults and clinical presentations between G. duodenalis genotypes. They are useful in understanding the transmission of G. duodenalis in humans in Spain.