文献类型：期刊文献

英文题名：Investigating the potential origin and formation of humic substances in biological wastewater treatment systems from the forms of phosphorus

作者：Chen, Mengfan[1];Jin, Xibiao[1];Wang, Yuan[1];Bao, Haojie[1]

机构：[1]East China Univ Sci & Technol, Res Inst Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Room 329,Expt Bldg 8,130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：42

期号：13

起止页码：1979

外文期刊名：ENVIRONMENTAL TECHNOLOGY

收录：;EI(收录号：20203309046386);WOS:【SCI-EXPANDED(收录号:WOS:000495158800001)】；

基金：This study was financially supported by the National Environmental Protection Public Welfare Science and Technology Research Program of China (No. 201309047).

语种：英文

外文关键词：Element analysis; humic substances; microbial cell debris; origin; P-31-NMR

摘要：Origin of the humic substances formed in biological wastewater treatment system (abbreviated as bio-HS) still remains inconclusive. In this study, the bio-HS that contained humic acid (HA) and fulvic acid (FA) from effluent and activated sludge were isolated and purified with XAD-8 resin and ion exchange resin, and then the molecular weight, functional groups, contents and forms of phosphorus (P) using gel permeation chromatography (GPC), fourier transform infrared (FTIR), element analysis and P nuclear magnetic resonance (P-31-NMR) were investigated. The results showed that HS was formed in biological wastewater treatment systems, and had a lower degree of humification than soil, peat, or marine HS, and mainly comprised diester and monoester P fragments. Specific signal peaks of intracellular materials and cell membranes (nucleic acid, phospholipids, and sugar phosphate) showed that microbial cell debris was the precursor of bio-HS. HA comprised diester P fragments and monoester P fragments that formed when diester P fragments degraded, while FA contained only diester P fragments. The monoester P fragments in HA may result from microbial degradation of diester P fragments, and FA may simultaneously condense into HA. These results show that microbial cell debris was first transformed into FA and then into HA. [GRAPHICS] .