文献类型：期刊文献

英文题名：Efficient Immobilization of Bacterial GH Family 46 Chitosanase by Carbohydrate-Binding Module Fusion for the Controllable Preparation of Chitooligosaccharides

作者：Lin, Si[1,2];Qin, Zhen[1,2];Chen, Qiming[1,2];Fan, Liqiang[1,2];Zhou, Jiachun[1,2];Zhao, Liming[1,2]

机构：[1]East China Univ Sci & Technol, R&D Ctr Separat & Extract Technol Fermentat Ind, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]SCICBT, Shanghai 200237, Peoples R China

年份：2019

卷号：67

期号：24

起止页码：6847

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20192407042101);WOS:【SCI-EXPANDED(收录号:WOS:000472679000019)】；

基金：This work was financially supported by the National Natural Science Foundation of China (31701537), the "Shu Guang" (15SG28) and "Chen Guang" (17CG28) Project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation, Shanghai Sailing Program (17YF1403500), Fundamental Research Funds for the Central Universities (222201814031, 22221818014), The National Key Research and Development Program of China (2018YFC0311200), and the 111 Project (B18022).

语种：英文

外文关键词：chitosanase; carbohydrate binding module; chitooligosaccharide; one-step immobilization-purification; packed-bed reactor

摘要：Chitooligosaccharide has been reported to possess diverse bioactivities. The development of novel strategies for obtaining optimum degree of polymerization (DP) chitooligosaccharides has become increasingly important. In this study, two glycoside hydrolase family 46 chitosanases were studied for immobilization on curdlan (insoluble beta-1,3-glucan) using a novel carbohydrate binding module (CBM) family 56 domain from a beta-1,3-glucanase. The CBM56 domain provided a spontaneous and specific sorption of the fusion proteins onto a curdlan carrier, and two fusion enzymes showed increased enzyme stability in comparison with native enzymes. Furthermore, a continuous packed-bed reactor was constructed with chitosanase immobilized on a curdlan carrier to control the enzymatic hydrolysis of chitosan. Three chitooligosaccharide products with different molecular weights were prepared in optimized reaction conditions. This study provides a novel CBM tag for the stabilization and immobilization of enzymes. The controllable hydrolysis strategy offers potential for the industrial-scale preparation of chitooligosaccharides with different desired DPs.