文献类型：期刊文献

英文题名：ArnB mediates CAMP resistance and in vivo colonization in the fish pathogen Edwardsiella piscicida

作者：Zhang, Riyu[1];Zhang, Yuanxing[2,3];Choi, Sang Ho[5];Wang, Qiyao[1,2,3];Song, Wei[4];Shao, Shuai[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[3]Lab Aquat Anim Dis MOA, Shanghai 200237, Peoples R China;[4]Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai, Peoples R China;[5]Seoul Natl Univ, Dept Agr Biotechnol, Natl Res Lab Mol Microbiol & Toxicol, Seoul, South Korea

年份：2023

卷号：576

外文期刊名：AQUACULTURE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001137322300001)】；

基金：This work was supported by grants from the National Key Research and Development Program (2022YFE0101200), the National Natural Science Foundation of China (32002436), and the China Agriculture Research System of MOF and MARA (CARS-47).

语种：英文

外文关键词：arnB; CAMP resistance; Edwardsiella piscicida; Fish pathogen; In vivo colonization

摘要：Adoption of a live attenuated vaccine (LAV) will be an effective disease management strategy in farmed fish. Given the natural existence of cationic antimicrobial peptides (CAMP) in fish, the CAMP susceptible strain has been a promising LAV candidate against the bacterial disease of fish. Edwardsiella piscicida, the aetiological agent of fish edwardsiellosis, has caused massive economic losses in aquaculture industries. However, the mechanism underlying the CAMP resistance of E. piscicida is not entirely clear. In this study, the arnB gene was identified to contribute to E. piscicida resistance to CAMP, such as polymyxin B and polymyxin E. The expression of arnB was inhibited by Mg2+ ions and activated by PhoP. Moreover, Delta arnB had impaired capability for in vivo colonization and had reduced virulence in turbot. Together, our findings demonstrated that arnB was required for CAMP resistance and in vivo colonization in E. piscicida, suggesting that Delta arnB might be a prospective potential LAV candidate against edwardsiellosis.