文献类型：期刊文献

英文题名：Discovery of a novel acrylic acid formation pathway in Gluconobacter oxydans and its application in biosynthesis of acrylic acid from glycerol

作者：Zhao, Li[1];Zhu, Jiawei[1];Ro, Kum-Song[1];Xie, Jingli[1,2];Wei, Dongzhi[1,2]

机构：[1]East China Univ Sci & Technol, Inst New World Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg SCICB, Shanghai 200237, Peoples R China

年份：2022

卷号：118

起止页码：182

外文期刊名：PROCESS BIOCHEMISTRY

收录：;EI(收录号：20221912077235);WOS:【SCI-EXPANDED(收录号:WOS:000860796900009)】；

基金：This work is supported by National Key Research and Development Program of China (Nos. 2020YFA0907800 and 2021YFC2100300) P. R. China.

语种：英文

外文关键词：Acrylic acid; Glycerol; Gluconobacter oxydans; Escherichia coli; Metabolic pathway; Biosynthesis

摘要：This study revealed a novel metabolic pathway of acrylic acid in Gluconobacter oxydans. First, the intermediate metabolite acrolein was confirmed by HPLC and GC-MS analyses. Then, the enzymes involved were identified based on mRNA expression analysis and gene deletion studies. This novel pathway includes three successive reactions: i) an alcohol dehydrogenase catalyzes 1,3-propanediol to 3-hydroxypropionaldehyde; ii) two isozymes, 3-hydroxyacyl-(ACP) dehydratase and 3-hydroxydecanoyl-(ACP) dehydratase convert 3-hydroxypropionaldehyde to acrolein; iii) an aldehyde dehydrogenase oxidizes acrolein to acrylic acid. An engineered Escherichia coli strain, coexpressing the glycerol dehydratase of Klebsiella pneumoniae and the 3-hydroxyacyl (ACP) dehydratase of G. oxydans was constructed for acrylic acid production from glycerol. The recombinant strain produced 144.0 +/- 9.8 mg/L of acrylic acid in a shake-flask culture. This is the first report demonstrating the biosynthetic pathway of acrylic acid via acrolein, and it provides a new approach for acrylic acid biosynthesis.