文献类型：期刊文献

英文题名：Metabolomic changes and metabolic responses to expression of heterologous biosynthetic genes for lycopene production in Yarrowia lipolytica

作者：Zhao, Chen[1,2];Gao, Qi[1];Chen, Jun[1];Wei, Liujing[1];Imanaka, Tadayuki[1];Hua, Qiang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Northwest A&F Univ, Coll Life Sci, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：251

起止页码：174

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20171903650293);WOS:【SCI-EXPANDED(收录号:WOS:000402470300024)】；

基金：This work was financially supported by National Natural Science Foundation of China (21576089), and National Basic Research Program of China (973 Program) (2012CB721101).

语种：英文

外文关键词：Yarrowia lipolytica; Metabolomics; Pathway abundance; Lipid; Lycopene

摘要：The metabolomic studies were conducted for both the engineered lycopene-producing Yarrowia lipolytica strain and the control strain. The changes in metabolome profiling and main pathway abundance of Y. lipolytica with the introduction of heterologous crt genes were investigated by multiple analytical and statistical methods More importantly, the metabolome data were transformed into pathway abundances visualized by a pathway relationship network using Cytoscape software. This network provided a simple way to present the statistical results of pathway abundance and interpret the effects of the crt genes in a metabolic view, and the results highlighted the up-regulation of eight pathways and the down-regulation of three pathways in the lycopene fast production phase. The comprehensive analysis suggested that the synthesis of lycopene has remarkable effects on fructose and mannose metabolism, citrate cycle, glyoxylate and dicarboxylate metabolism, glycerophospholipid metabolism as well as the biosynthesis and metabolism of some amino acids including valine, leucine and isoleucine in Y. lipolytica.