文献类型：期刊文献

英文题名：Cloning and characterization of a novel tyrosine ammonia lyase-encoding gene involved in bagremycins biosynthesis in Streptomyces sp.

作者：Zhu, Yunxia[1];Liao, Siyi[1];Ye, Jiang[1];Zhang, Huizhan[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2012

卷号：34

期号：2

起止页码：269

外文期刊名：BIOTECHNOLOGY LETTERS

收录：;EI(收录号：20120514727897);WOS:【SCI-EXPANDED(收录号:WOS:000299510200012)】；

语种：英文

外文关键词：Aromatic amino acid ammonia lyase; Bagremycins; trans-coumaric acid; Streptomyces; Tyrosine ammonia lyase

摘要：Tyrosine ammonia lyase catalyzes the deamination of l-tyrosine to trans-coumaric acid. A novel tyrosine ammonia lyase-encoding gene, bagA, was cloned and sequenced from bagremycins-producing strain Streptomyces sp. Tu 4128 whose protein product contains a Ala-Ser-Gly segment in the active site. The disruption of the bagA gene abolished trans-coumaric acid and bagremycins production. trans-coumaric acid restored the formation of bagremycin A in the mutant, but not bagremycin B. Thus, trans-coumaric acid is a precursor for biosynthesis of bagremycins and the bagA gene codes for tyrosine ammonia lyase to synthesize trans-coumaric acid. This is a novel bacterial tal gene reported in actinomycetes for the second time and for the first time in a Streptomyces sp.