文献类型：期刊文献

英文题名：Nicandra physalodes Extract Exerts Antiaging Effects in Multiple Models and Extends the Lifespan of Caenorhabditis elegans via DAF-16 and HSF-1

作者：Wang, Jiqun[1];Huang, Yunyuan[2];Shi, Kaixuan[3];Bao, Lingyuan[1];Xiao, Chaojiang[3];Sun, Tianyue[1];Mao, Zhifan[1];Feng, Jiali[1];Hu, Zelan[1];Guo, Zhenghan[3];Li, Jing[3];Jiang, Bei[3];Liu, Wenwen[4];Li, Jian[1,3,4,5]

机构：[1]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Frontiers Sci Ctr Materiobiol & Dynam Chem,Shangha, Sch Pharm,State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China;[3]Dali Univ, Coll Pharm, Yunnan Key Lab Screening & Res Antipathogen Plant, Dali 671000, Yunnan, Peoples R China;[4]Hainan Univ, Coll Pharm, Key Lab Trop Biol Resources, Minist Educ, Haikou 570228, Hainan, Peoples R China;[5]Tongji Univ, Shanghai Peoples Hosp 10, Clin Med Sci & Tech Innovat Ctr, Sch Med, Shanghai 200092, Peoples R China

年份：2022

卷号：2022

外文期刊名：OXIDATIVE MEDICINE AND CELLULAR LONGEVITY

收录：;EI(收录号：20224413050056);WOS:【SCI-EXPANDED(收录号:WOS:000876505200010)】；

基金：AcknowledgmentsWe thank the Caenorhabditis Genetics Center (CGC) for providing the worm strains. Financial support for this research was provided by the National Natural Science Foundation of China (22037002, 82104068, and 81872747), the Innovative Research Team of High-Level Local Universities in Shanghai (SHSMU-ZDCX20212702), the National Special Fund for State Key Laboratory of Bioreactor Engineering (2060204), the Innovation Program of Shanghai Municipal Education Commission (2021-01-07-00-02-E00104), and the Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (2021 Sci & Tech 03-28).

语种：英文

外文关键词：Cell culture - Diagnosis - Mammals - Plant extracts

摘要：The development of safe and effective therapeutic interventions is an important issue for delaying aging and reducing the risk of aging-related diseases. Chinese herbal medicines for the treatment of aging and other complex diseases are desired due to their multiple components and targets. Through screening for effects on lifespan of 836 Chinese herbal medicine extracts, Nicandra physalodes extract (HL0285) was found to exhibit lifespan extension activity in Caenorhabditis elegans (C. elegans). In further experiments, HL0285 improved healthspan, enhanced stress resistance, and delayed the progression of neurodegenerative diseases in C. elegans. Additionally, it ameliorated senescence in human lung fibroblasts (MRC-5 cells) and reversed liver function damage and reduced senescence marker levels in doxorubicin- (Dox-) induced aging mice. In addition, the longevity effect of HL0285 in C. elegans was dependent on the DAF-16 and HSF-1 signaling pathways, as demonstrated by the results of the mutant lifespan, gene level, and GFP level assays. In summary, we discovered that HL0285 had an antiaging effect in C. elegans, MRC-5 cells, and Dox-induced aging mice and deserves to be explored in the future studies on antiaging agents.