文献类型：期刊文献

英文题名：Extraction and isolation of diphenylheptanes and flavonoids from Alpinia officinarum Hance using supercritical fluid extraction followed by supercritical fluid chromatography

作者：Wu, Di[1];Ge, Dandan[1];Dai, Yingping[1];Chen, Yanchun[1];Fu, Qing[1,2];Jin, Yu[1,2]

机构：[1]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Shanghai 200237, Peoples R China

年份：2023

卷号：46

期号：14

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20232014092290);WOS:【SCI-EXPANDED(收录号:WOS:000985727600001)】；

语种：英文

外文关键词：Alpinia officinarum Hance; diphenylheptanes; flavonoids; supercritical fluid chromatography; supercritical fluid extraction

摘要：In this paper, an off-line combination method of supercritical fluid extraction and supercritical fluid chromatography was developed for the selective extraction and isolation of diphenylheptanes and flavonoids from Alpinia officinarum Hance. The enrichment of target components was successfully achieved using supercritical fluid extraction with the following conditions (8% ethanol as co-solvent at 45 degrees C and 30 MPa for 30 min). Taking full advantage of the complementarity of supercritical fluid chromatography stationary phases, a two-step preparative supercritical fluid chromatography strategy was constructed. The extract was firstly divided into seven fractions on a Diol column (250 x 20 mm internal diameter, 10 mu m) within 8 min by gradient elution increasing from 5% to 20% modifier (methanol) at 55 ml/min and 15 MPa. Then the seven fractions were separated by using a 1-AA or a DEA column (250 x 19 mm internal diameter, 5 mu m) at 50 ml/min and 13.5 MPa. This two-step strategy showed superior separation ability for structural analogs. As a result, seven compounds, including four diphenylheptanes and three flavonoids with high purity, were successfully obtained. The developed method is also helpful for the extraction and isolation of other structural analogs of traditional Chinese medicines.