文献类型：期刊文献

英文题名：Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production

作者：Bai, Fangmin[1];Dai, Lu[1];Fan, Jiying[1];Ngoctu Truong[1];Rao, Ben[1];Zhang, Liaoyuan[2];Shen, Yaling[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Fujian Agr & Forestry Univ, Coll Life Sci, Minist Educ, Key Lab Biopesticide & Chem Biol, Fuzhou 350002, Fujian, Peoples R China

年份：2015

卷号：42

期号：5

起止页码：779

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242616495195);WOS:【SCI-EXPANDED(收录号:WOS:000352860600012)】；

基金：This work was supported by Shanghai Leading Academic Discipline Project (project B505) and National Special Fund for State Key Laboratory of Bioreactor Engineering (no. 2060204).

语种：英文

外文关键词：(3R)-acetoin; (2R, 3R)-2,3-butanediol; Meso-2,3-butanediol dehydrogenase; (2R,3R)-2,3-butanediol dehydrogenase; Serratia marcescens

摘要：(3R)-Acetoin and (2R,3R)-2,3-butanediol are important pharmaceutical intermediates. However, until now, the quantity of natural microorganisms with the ability to produce single configuration of optically pure (3R)-acetoin and (2R,3R)-2,3-butanediol is rare. In this study, a meso-2,3-butanediol dehydrogenase encoded by the slaC gene from Serratia marcescens MG1 was identified for meso-2,3-butanediol and (2S,3S)-2,3-butanediol biosynthesis. Inactivation of the slaC gene could significantly decrease meso-2,3-butanediol and (2S,3S)-2,3-butanediol and result in a large quantity of (3R)-acetoin accumulation. Furthermore, a (2R,3R)-2,3-butanediol dehydrogenase encoded by the bdhA gene from Bacillus subtilis 168 was introduced into the slaC mutant strain of Serratia marcescens MG1. Excess (2R,3R)-2,3-butanediol dehydrogenase could accelerate the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol and lead to (2R,3R)-2,3-butanediol accumulation. In fed-batch fermentation, the excess (2R,3R)-2,3-butanediol dehydrogenase expression strain could produce 89.81 g/l (2R,3R)-2,3-butanediol with a productivity of 1.91 g/l/h at 48 h. These results provided potential applications for (3R)-acetoin and (2R,3R)-2,3-butanediol production.