文献类型：期刊文献

英文题名：Three-dimensional bioprinting is not only about cell-laden structures

作者：Zhang, Hong-Bo[1];Xing, Tian-Long[1];Yin, Rui-Xue[1];Shi, Yong[1];Yang, Shi-Mo[1];Zhang, Wen-Jun[1,2]

机构：[1]East China Univ Sci & Technol, Sch Mech & Power Engn, Complex & Intelligent Res Ctr, Shanghai, Peoples R China;[2]Univ Saskatchewan, Div Biomed Engn, Saskatoon, SK S7N 5A9, Canada

年份：2016

卷号：19

期号：4

起止页码：187

外文期刊名：CHINESE JOURNAL OF TRAUMATOLOGY

收录：WOS:【ESCI(收录号:WOS:000442109000001)】；

语种：英文

外文关键词：3D bioprinting; Hydrogels; Vascularization; Multiple cell lines

摘要：In this review, we focused on a few obstacles that hinder three-dimensional (3D) bioprinting process in tissue engineering. One of the obstacles is the bioinks used to deliver cells. Hydrogels are the most widely used bioink materials; however, they are mechanically weak in nature and cannot meet the requirements for supporting structures, especially when the tissues, such as cartilage, require extracellular matrix to be mechanically strong. Secondly and more importantly, tissue regeneration is not only about building all the components in a way that mimics the structures of living tissues, but also about how to make the constructs function normally in the long term. One of the key issues is sufficient nutrient and oxygen supply to the engineered living constructs. The other is to coordinate the interplays between cells, bioactive agents and extracellular matrix in a natural way. This article reviews the approaches to improve the mechanical strength of hydrogels and their suitability for 3D bioprinting; moreover, the key issues of multiple cell lines coprinting with multiple growth factors, vascularization within engineered living constructs etc. were also reviewed. (C) 2016 Daping Hospital and the Research Institute of Surgery of the Third Military Medical University.