文献类型：期刊文献

英文题名：Doxorubicin hydrochloride-oleic acid conjugate loaded nanostructured lipid carriers for tumor specific drug release

作者：Zhao, Shuangni[1,2];Le Van Minh[3,4,8];Li, Na[5,6];Gararnus, Vasil M.[7];Handge, Ulrich A.[7];Liu, Jianwen[3,4];Zhang, Rongguang[5,6];Willumeit-Roemer, Regine[7];Zou, Aihua[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China;[5]Natl Ctr Prot Sci Shanghai, Shanghai 200237, Peoples R China;[6]Shanghai Inst Biochem & Cell Biol, Shanghai 200237, Peoples R China;[7]Helmholtz Zentrum Geesthacht, Ctr Mat & Coastal Res, Inst Mat Res, Max Planck Str 1, D-21502 Geesthacht, Germany;[8]Nguyen Tat Thanh Univ, NIT Hi Technol Inst, Dist 4, Ho Chi Minh City, Vietnam

年份：2016

卷号：145

起止页码：95

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;EI(收录号：20162402480690);WOS:【SCI-EXPANDED(收录号:WOS:000381171800013)】；

基金：We gratefully acknowledge the support of this work by the Shanghai Natural Science Foundation (Grant No. 15ZR1409900), the National Natural Science Foundation of China (Grant No. 21573070), Fundamental Research Funds for the Central Universities, and the Knowledge Innovation Program of CAS (Grant No. 2013KIP103). We thank the staff of the BL19U2 beamline at the National Center for Protein Science Shanghai and the Shanghai Synchrotron Radiation Facility (Shanghai, People's Republic of China) for assistance during data collection. We also thank Mrs. Ivonne Ternes for the DSC measurements.

语种：英文

外文关键词：DOX; Oleic acid; NLCs; SAXS; pH-dependent drug release

摘要：The hydrophilic drug Doxorubicin hydrochloride (DOX) paired with oleic acid (OA) was successfully incorporated into nanostructured lipid carriers (NLCs) by a high-pressure homogenization (HPH) method. Drug nanovehicles with proper physico-chemical characteristics (less than 200nm with narrow size distribution, spherical shape, layered internal organization, and negative electrical charge) were prepared and characterized by dynamic light scattering, zeta potential measurements, transmission electron microscopy, small-angle X-ray scattering and differential scanning calorimetry. The drug loading and entrapment efficiency of DOX-OA/NLCs were 4.09% and 97.80%, respectively. A pH-dependent DOX release from DOX-OA/NLCs, i.e., fast at pH 3.8 and 5.7 and sustained at pH 7.4, was obtained. A cytotoxicity assay showed that DOX-OA/NLCs had comparable cytotoxicity to pure DOX and were favorably taken up by HCT 116 cells. The intracellular distribution of DOX was also studied using a confocal laser scanning microscope. All of these results demonstrated that DOX-OA/NLCs could be a promising drug delivery system with tumor-specific DOX release for cancer treatment. (C) 2016 Elsevier B.V. All rights reserved.