文献类型：期刊文献

中文题名：铽基金属有机框架材料对过氧化氢酶的固定化及其性能评价

英文题名：Immobilization of catalase by terbium-based metal organic frameworks and evaluation on its performance

作者：孙烨琳[1];何义[1];李杰[1];张维冰[1];张磊[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2025

卷号：45

期号：7

起止页码：160

中文期刊名：现代化工

外文期刊名：Modern Chemical Industry

收录：;北大核心:【北大核心2023】；

基金：国家重点研发计划(2021YFF0701900);上海市重点实验室经费(JKJ01241717)。

语种：中文

中文关键词：过氧化氢酶;固定化酶;金属有机框架材料;电子顺磁共振波谱

外文关键词：catalase;immobilized enzyme;MOFs;electron paramagnetic resonance spectroscopy

摘要：开发了一种在铽基金属有机框架材料(Tb-MOF)中封装过氧化氢酶(CAT)制备固定化酶材料(CAT@Tb-MOF)的方法,制备的固定化酶材料负载量高达302.3 mg/g。采用紫外-可见分光光度法和电子顺磁共振波谱法(EPR)对封装后的过氧化氢酶活性加以评价,结果发现其酶活保留率达90%以上,且具有良好的环境适应性和重复使用性。MOFs材料在生长过程中直接将酶包覆在其中,具有条件温和以及操作便捷的特点。在固定化过程中酶结构保持稳定,因此可以良好地保持酶活性,同时MOFs材料提供的微环境也保证了酶的环境适应性。在80℃下剩余酶活为92.6%;经过10次循环使用后,固定化酶活性仍能保持在90%;经过50 d贮藏,固定化酶活性仍保持在50%左右。该方法所制备的固定化酶材料具有良好的稳定性,在工业应用领域具有广阔的应用潜力。
A method is developed for encapsulating catalase(CAT)in terbium-based metal organic framework(Tb-MOF)materials to prepare an immobilized catalase material(CAT@Tb-MOF).The loading capacity of the immobilized catalase material reaches as high as 302.3 mg/g.The activity of catalase encapsulated is evaluated by means of UV-visible spectrophotometry and electron paramagnetic resonance spectroscopy,respectively.It is indicated that the retention rate of catalase activity exceeds 90%,and the material has good environmental adaptability and reusability.MOFs materials encapsulate catalase directly during the growth process,which has the characteristics of mild conditions and convenient operation.The catalase remains stable structure in the immobilization process,so the activity of catalase can be maintained well while the microenvironment provided by MOFs materials also ensures the environmental adaptability of the catalase.The remaining activity of catalase is 92.6%at 80℃.The activity of immobilized catalase still maintains at 90%after 10 cycles,and at about 50%after 50 days of storage.The prepared CAT@Tb-MOF has good stability,showing broad application potential in the field of industrial application.