文献类型：期刊文献

中文题名：基于3D微流控芯片的水凝胶微胶囊可控生成系统

英文题名：A 3D Microfluidic-Based Hydrogel Microcapsule Fabrication System

作者：金聪[1];赵旭晟[2];郭嘉灏[1];欧阳立明[2];姜国俊[1];顾震[1];王慧锋[1]

机构：[1]华东理工大学信息科学与工程学院,上海200237;[2]华东理工大学生物工程学院,上海200237

年份：2025

卷号：51

期号：6

起止页码：783

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：;北大核心:【北大核心2023】；

基金：国家重大科研仪器研制项目(32327801)。

语种：中文

中文关键词：微纳加工;3D微流控芯片;液滴微流控;微胶囊;3D细胞培养

外文关键词：micro-nano fabrication;3D microfluidic chip;droplet microfluidics;microcapsule;3D cell culture

摘要：围绕水凝胶微胶囊制备需求,利用3D微纳打印在快速、低成本和高精度等方面的优势,发展了基于3D微流控芯片的微胶囊制备系统,该系统通过构建具有3D微结构的液滴微流控芯片,以双层鞘流生成微胶囊颗粒,并实现微胶囊核壳比例的可控生成。实验结果显示,利用3D微纳打印得到的模具,通过翻模制造微流控芯片,可达到高精度与高平整度,通道尺寸误差均小于2μm。所生成微胶囊的内、外径变异系数均小于3%且一致性优异。细胞增殖实验表明,所生成微胶囊可用于3D细胞培养,证实了所提出方法在规模化细胞培养及再生医学等领域具有广泛应用潜力。
To meet the demand for hydrogel microcapsule fabrication,this study aimed to develop a highthroughput system based on a 3D microfluidic chip for producing core-shell microcapsules.The system uses 3D micro-/nano-printing technology,which offers fast prototyping,low cost,and high precision.This method enables precise control over the structure of the microcapsules.The system includes a droplet microfluidic chip with 3D microstructures,which are fabricated using a dual-layer sheath flow strategy to generate the microcapsules.Experimental results show that the flow-focusing region on the inner bottom surface of the 3D micro-/nano-printed mold has a flatness of 4.65μm,and the dimensional errors in the three-phase channels are always less than 2μm.Similarly,the PDMS plate holding the microchannels shows a flatness of 4.81μm.The channel dimensional errors in the PDMS plate are also within 2μm.These results indicate that both the mold and the PDMS plate possess high flatness and accuracy,meeting the technical requirements for making microcapsules.Microcapsules with different inner diameters were prepared by changing the flow rate of the core solution(40,60,80,100,120μL/h),while the outer diameter remained nearly constant.The microcapsules produced by the system have a very low coefficient of variation(CV<3%)for both outer and inner diameters,showing that the capsules are very uniform in size.Finally,cell culture tests were done to evaluate the biocompatibility and functionality of the microcapsules.A549 cells showed clear growth and clustering after two days of culture inside the microcapsules,confirming that the microcapsules from this system support cell growth well.These results suggest that the system has great potential for large-scale applications in biomedical fields.