文献类型：期刊文献

英文题名：Effect of culture conditions on producing and uptake hydrogen flux of biohydrogen fermentation by metabolic flux analysis method

作者：Niu, Kun[1,2];Zhang, Xu[1];Tan, Wen-Song[1];Zhu, Ming-Long[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Inst Bioengn, Hangzhou 310032, Zhejiang, Peoples R China

年份：2011

卷号：102

期号：15

起止页码：7294

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20112414062875);WOS:【SCI-EXPANDED(收录号:WOS:000292668000009)】；

基金：The authors are grateful for the financial support of this research by the National Special Fund of China for State Key Laboratory of Bioreactor Engineering, Grant No. 2060204.

语种：英文

外文关键词：Klebsiella pneumoniae; Culture conditions; MFA; Producing hydrogen flux; Uptake hydrogen flux

摘要：In this work, metabolic flux analysis (M FA) method was used to estimate the effects of the culture conditions on both the producing and uptake hydrogen flux inside the cell of Klebsiella pneumoniae ECU-15. The results indicated that higher temperature could reduce the amount of the uptake hydrogen and enhance the hydrogen production from the NADH pathway. Moreover, both the producing hydrogen flux from formate and the uptake hydrogen flux were attained to the maximum at pH 7.0-7.5. The producing hydrogen flux was higher at 5 g/L initial glucose than that of the other concentrations, and the uptake hydrogen flux showed the minimum value under the same condition. The apparent hydrogen generation was caused by the combined action of producing hydrogenase, uptake hydrogenase and bidirectional hydrogenase. These results were helpful to deeply understand the mechanism of the biohydrogen evolving process and establish the suitable molecular strategies for improving hydrogen production. (C) 2011 Elsevier Ltd. All rights reserved.