文献类型：期刊文献

英文题名：An engineered quorum-sensing-based whole-cell biosensor for active degradation of organophosphates

作者：He, Jianwei[1];Zhang, Xiaoyan[1];Qian, Yuanyi[1];Wang, Qiyao[1];Bai, Yunpeng[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2022

卷号：206

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;EI(收录号：20220911736247);WOS:【SCI-EXPANDED(收录号:WOS:000782656400005)】；

基金：Acknowledgements This work was financially sponsored by the National Key Research and Development Program of China (2016YFA0204300) , National Natural Science Foundation of China (No. 22078096) , and Natural Science Foundation of Shanghai (18ZR1409900) .

语种：英文

外文关键词：Whole-cell biosensor; Synthetic biology; Genetic circuits; Quorum sensing; Degradation

摘要：The environmental accumulation of organophosphates is a serious threat to public health. To detect these xenobiotics, a broad range of sensors has been developed in past decades. However, sensors with high sensitivity and a capability for degrading organophosphates are rare. In this study, "smart" whole-cell biosensors were created by combining synthetic biology approaches with the bacterial quorum sensing (QS) mechanism. The engineered whole-cell biosensor pUC57-QS-DSF-F42 L/E coli DH5 alpha can sense a wide array of phenolic compounds including phenol and p-nitrophenol (p-NP). By optimizing the genetic circuits, the phenol and p-NP detection limits reached 0.1 and 1 mu M, respectively. Importantly, by replacing the fluorescence-generated reporter sfGFP with MP-degrading enzyme PoOPHM2, the whole-cell biosensor pUC57-OPH-QS-DSF-F42 L/E coli DH5 alpha actively degraded 10 and 100 mu M methyl parathion (MP), a typical organophosphate pesticide, which was artificially added to the cell culture at different time points in five consecutive degrading experiments, demonstrating its MP sensing and degrading capabilities. The universal design of this new biosensor can be used to create more efficient biosensors to detect and degrade various pollutants in the environment for rapid testing and bioremediation.