文献类型：期刊文献

英文题名：Hydrogenase as the basis for green hydrogen production and utilization

作者：Ji, Haishuo[1];Wan, Lei[2];Gao, Yanxin[3];Du, Ping[3];Li, Wenjin[3];Luo, Hang[3];Ning, Jiarui[3];Zhao, Yingying[3];Wang, Huangwei[4,5];Zhang, Lixin[4,5];Zhang, Liyun[1,3]

机构：[1]Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300350, Peoples R China;[2]Max Planck Inst Chem Energy Convers, Stiftstr 34-36, D-45470 Mulheim, Germany;[3]Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin 300350, Peoples R China;[4]East China Univ Sci & Technol ECUST, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol ECUST, Sch Biotechnol, Shanghai 200237, Peoples R China

年份：2023

卷号：85

起止页码：348

外文期刊名：JOURNAL OF ENERGY CHEMISTRY

收录：;EI(收录号：20233214509750);WOS:【SCI-EXPANDED(收录号:WOS:001062627500001)】；

基金：This work was supported by the National Key Research and Development Program of China (Nos. 2020YFA0907300, 2020YFA0907800) , the National Natural Science Foundation of China (No. 22077069) , the Natural Science Foundation of Tianjin (Nos. 19JCZDJC33400 and 21JCYBJC00310) , and the Fundamental Research Funds for the Central Universities, Nankai University (No. 63201111) .

语种：英文

外文关键词：Hydrogenase; Biological H-2 energy; Oxygen tolerance; Artificial photosynthesis; Biofuel cells

摘要：Hydrogenase isa paradigm of highly efficient biocatalyst for H2 production and utilization evolved in nature. A dilemma is that despite the high activity and efficiency expected for hydrogenases as promising catalysts for the hydrogen economy, the poor oxygen tolerance and low yield of hydrogenases largely hinder their practical application. In these years, the enigmas surrounding hydrogenases regarding their structures, oxygen tolerance, mechanisms for catalysis, redox intermediates, and proton-coupled electron transfer schemes have been gradually elucidated; the schemes, which can well couple hydrogenases with other highly efficient (in)organic and biological catalysts to build novel reactors and drive valuable reactions, make it possible for hydrogenases to find their niches. To see how scientists put efforts to tackle this issue and design novel reactors in the fields where hydrogenases play crucial roles, in this review, recent advances were summarized, including different strategies for protecting enzyme molecules from oxygen, enzyme-based assembling systems for H2 evolution in the photoelectronic catalysis, enzymatic biofuel cells for H2 utilization and storage and the efficient electricity-hydrogen-carbohydrate cycle for high-purity hydrogen and biofuel automobiles. Limitations and future perspectives of hydrogenasebased applications in H2 production and utilization with great impact are discussed. In addition, this review also provides a new perspective on the use of biohydrogen in healthcare beyond energy. (c) 2023 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by ELSEVIER B.V. and Science Press. All rights reserved.