文献类型：期刊文献

英文题名：Self-Assembly of a Monochromophore-Based Polymer Enables Unprecedented Ratiometric Tracing of Hypoxia

作者：Wang, Shuwen[1,3,4];Gu, Kaizhi[1,3,4];Guo, Zhiqian[1,3,4];Yan, Chenxu[1,3,4];Yang, Tingyuan[2,5];Chen, Zhuo[2,5];Tian, He[1,3,4];Zhu, Wei-Hong[1,3,4]

机构：[1]Shanghai Key Lab Funct Mat Chem Key Lab Adv Mat &, E China Univ Sci & Technol, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Key Lab Adv Mat, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Joint Int Res Lab Precis Chem & Mol Engn, Feringa Nobel Prize Scientist Joint Res Ctr, Inst Fine Chem,Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[5]East China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2019

卷号：31

期号：3

外文期刊名：ADVANCED MATERIALS

收录：;EI(收录号：20184906174661);WOS:【SCI-EXPANDED(收录号:WOS:000459628200013)】；

基金：S.W. and K.G. contributed equally to this work. This work was supported by NSFC Science Center Program (21788102) and Creative Research Groups (21421004), National Key Research and Development Program (2016YFA0200300 and 2017YFC0906902), NSFC/China (21636002 and 21622602), National Postdoctoral Program for Innovative Talents (BX201700075), and Program of Introducing Talents of Discipline to Universities (B16017). Human lung carcinoma epithelial A549 cells were supplied by Institute of Cell Biology (Shanghai, China). This study was performed in strict accordance with the NIH guidelines for the care and use of laboratory animals (NIH Publication No. 85-23 Rev. 1985), and approved by the Institutional Animal Care and Use Committee of the National Tissue Engineering Center (Shanghai, China).

语种：英文

外文关键词：clustering-triggered emission; hypoxia sensing; monochromophore-based polymers; NIR ratiometric mode; self-assembly

摘要：The accuracy of traditional bischromophore-based ratiometric probes is always compromised by undesirable energy/charge transferring interactions between the internal reference moiety and the sensing chromophore. In this regard, ratiometric sensing with a monochromophore system is highly desirable. Herein, an unprecedented monochromophore-based ratiometric probe, which consists of a hydrophilic backbone poly(N-vinylpyrrolidone) (PVP) and single chromophore of platinum(II) tetraphenylporphyrin (Pt-TPP) is reported. Combination of the specific assembled clustering-triggered fluorescent emission (oxygen-insensitive) with the original Pt-TPP phosphorescence (oxygen-sensitive) enables successful construction of a monochromophore-based ratiometric nanosensor for directly tracing hypoxia in vivo, along with the preferable facilitation of enhanced permeation and retention effect and long excitation wavelength. The unique ratiometric signals enable the direct observation from normoxic to hypoxic environment in both living A549 cells and a tumor-bearing mice model, providing a significant paradigm of a monochromophore-based dual-emissive system with the specific assembled cluster emission. The work satisfactorily demonstrates a valuable strategy for designing monochromophore-based dual-emissive materials, and validates its utility for in vivo ratiometric biological sensing without the common energy/charge interference in bischromophore-based system.