文献类型：期刊文献

中文题名：重组粘质沙雷氏菌混合发酵木糖-葡萄糖生产2,3-丁二醇

英文题名：Production of 2,3-Butanediol from Xylose and Glucose by Recombinant Serratia marcescens MG1

作者：盛文建[1];白方敏[1];戴璐[1];沈亚领[1]

机构：[1]华东理工大学生物工程学院

年份：2019

卷号：38

期号：12

起止页码：5475

中文期刊名：基因组学与应用生物学

外文期刊名：Genomics and Applied Biology

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：上海市重点学科建设资助项目(No.B505);生物反应器工程国家重点实验室重点(No.2060204)项目共同资助

语种：中文

中文关键词：木糖;2,3-丁二醇;重组粘质沙雷氏菌;混合发酵

外文关键词：Xylose;2,3-Butanediol;Engineered Serratia marcescens;Co-fermentation

摘要：本研究通过生物发酵的方法,可将木质纤维素水解液中的木糖代谢为广泛应用于化工、能源、食品和农业等领域的生物基产品-2,3-丁二醇。通过在粘质沙雷氏菌(Serratia marcescens)MG1中可溶性表达木糖异构酶基因(重组粘质沙雷氏菌),以及优化培养条件。在接种量为5%、温度为30℃、pH为6.0的条件下,该菌代谢木糖的浓度可增至30.5 g/L,且代谢得到2,3-丁二醇浓度可达到8.5 g/L,相比野生菌代谢木糖得到的2,3-丁二醇的浓度(4.2 g/L)提高了102.4%。此外,为了缩短重组粘质沙雷氏菌代谢木糖的周期,采用了木糖和葡萄糖混合发酵的方法,使生产周期从84 h降至72 h,且2,3-丁二醇产量为20.1 g/L,对混合糖的转化率为0.29 g/g。
In this study, bio-fermentation can be used to metabolize xylose from lignocellulosic hydrolysate to2,3-butanediol, a bio-based producthas been widely applied intothe fields of chemical, energy, food and agriculture.Through the soluble expression of the xylose isomerase gene(Serratia marcescens) in Serratia marcescens MG1 and optimization of culture conditions, with the inoculation amount, temperature and pH were 5%, 30℃ and 6.0,respectively, the concentration of xylose metabolized by the bacteria can be increased to 30.5 g/L, and the concentration of 2,3-butanediol generated by metabolism can be increased to 8.5 g/L, which is an increase of102.4% over that of the metabolism of xylose by wild bacteria.In addition, in order to shorten the period of recombinant xylose-metabolizing xylose, a co-fermentation of xylose and glucose was adopted, and the production periodwas reduced from 84 h to 72 h, and the output of 2,3-butanediol and conversion rate of the mixed sugar were 20.1 g/L and 0.29 g/g, respectively.