文献类型：期刊文献

英文题名：Enhancing nemadectin production by Streptomyces cyaneogriseus ssp noncyanogenus through quantitative evaluation and optimization of dissolved oxygen and shear force

作者：Song, Xiaoqing[1];Zhang, Yun[1];Xue, Jiayun[1];Li, Chao[1];Wang, Zejian[1];Wang, Yonghong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2018

卷号：255

起止页码：180

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20180704781115);WOS:【SCI-EXPANDED(收录号:WOS:000426216400024)】；

基金：This work was financially supported by grants from National High Technology Research and Development Program (2015AA021005).

语种：英文

外文关键词：Nemadectin; Oxygen supply; Shear stress; Impeller configuration; Computational fluid dynamics

摘要：In this study, effects of oxygen supply and shear stress on nemadectin production by Streptomyces cyaneogriseus ssp. noncyanogenus (S. cyaneogriseus) fermentation were investigated in shake flasks and 5-L bioreactors. Results showed sufficient dissolved oxygen level was essential for cells growth and nemadectin biosynthesis, while strong shear stress had negative impacts on both cell growth and nemadectin synthesis. Furthermore, when a combined paddle type was applied in culturing S. cyaneogriseus, the nemadectin production was increased by 23.6%. The influence of different agitation rates and paddle types on volumetric oxygen transfers coefficient (KLa) and shear stress were quantitatively studied through computational fluid dynamics simulation (CFD). The results of CFD revealed that high KLa as well as low shear stress co-existed under the combined impeller configuration at 650 rpm. This study is expected to be helpful to the scale-up of nemadectin fermentation and other stress-sensitive but high-oxygen-consumption filamentous microorganism.