文献类型：期刊文献

中文题名：A highly sensitive fluorescent probe for tracking intracellular zinc ions and direct imaging of prostatic tissue in mice

作者：Yuyu Wang[1];Houna Duan[1];Hongyuan Shi[2];Shiwei Zhang[1];Yufang Xu[1];Weiping Zhu[1];Xuhong Qian[1]

机构：[1]State Key Laboratory of Bioreactor Engineering,Shanghai Key Laboratory of Chemical Biology,School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China;[2]Department of Radiology,Jiangsu Province Hospital,First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China

年份：2020

卷号：31

期号：11

起止页码：2933

中文期刊名：Chinese Chemical Letters

外文期刊名：中国化学快报（英文版）

收录：CSTPCD;;Scopus;CSCD:【CSCD2019_2020】；PubMed;

基金：the National Natural Science Foundation of China(Nos.21878088,81701760,21476077);Key Project of the Shanghai Science and Technology Committee(No.18DZ1112703);Science and Technology Project of Jiangsu Province of China(No.BK20171086)for financial support。

语种：英文

中文关键词：Fluorescent probe;Zinc ion;Prostate cells;Naphthalimide;In vivo imaging

摘要：A highly sensitive fluorescent sensor ZnDN was designed,synthesized and used for tracking intracellular zinc ions in various living cells and direct imaging of prostatic tissue in mice.ZnDN was prepared from the heterocyclic-fused naphthalimide fluorophore,and the zinc receptor,N,N-bis(2-pyridylmethyl)ethylenediamine(BPEN).Upon addition of Zn2+to the solutions of ZnDN,a remarkable fluorescence enhancement was observed,which could be attributed to the photo-induced electron transfer(PET)mechanism.Since ZnDN exhibited high sensitivity toward Zn2+in phosphate buffer solution,with a limit of detection of 4.0 x 10-9 mol/L,it was further applied for the imaging of exogenous and endogenous Zn21 in different living cells.Living cells imaging experiments suggested that ZnDN could image the changes of intracellular free zinc ions,and could be used for two-photon imaging.Moreover,flow cytometry suggested that ZnDN could distinguish cancerous prostate cells from normal cells.Animal experiments indicated that ZnDN had the potential in imaging prostate tissue in vivo.