文献类型：期刊文献

英文题名：Identification of characteristic microbes and metabolites in confined environments population

作者：Feng, Xiangru[1];Zhong, Jiaqian[2];Wang, Jiaming[2];Lu, Xiaoxuan[1];Chen, Yijia[1];Yao, Yanying[2];Ji, Xiaoguo[1,3];Zhao, Mengyao[1,3];Jin, Jiayang[1,4];Li, Juan[2];Zhao, Liming[1,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Naval Med Univ, Changzheng Hosp, Dept Nutr, Shanghai, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, Shanghai, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol SCI, Shanghai, Peoples R China

年份：2025

卷号：130

外文期刊名：BRAIN BEHAVIOR AND IMMUNITY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001582378200002)】；

基金：This work was supported by the Natural Science Foundation of Shanghai (23ZR1415400) , Shanghai Frontiers Science Center of Opto-genetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission) and Shanghai Post-doctoral Excellence Program (2022153) .

语种：英文

外文关键词：Confined environments; Stress; Emotional states; Gut microbiota; Metabolomics

摘要：Chronic stress in confined environments can disrupt the microbiota-gut-brain axis. However, the characteristic microbial and metabolic alterations in socially and radically basic activity-restricted people in confined environments (SRBARC) remain unclear. This study integrated assessments of emotional states, gut microbiota, and metabolomic profiles to investigate their interrelationships in SRBARC, aiming to identify characteristic microbes and metabolites. The results suggested that the SRBARC tend to exhibit depressive/anxiety-like behaviors, accompanied by hypothalamic-pituitary-adrenal (HPA) axis activation, suppressed testosterone, and elevated pro-inflammatory cytokines. Full-length 16S rDNA sequencing analysis indicated lower Shannon and Chao1 indices in the gut microbiota of the SRBARC. Random forest analysis combined with dual-cohort verification suggested that Blautia massiliensis, Blautia wexlerae, Coprococcus comes, Faecalibacterium prausnitzii, and Lachnospiraceae bacterium may serve as characteristic microbes with diagnostic value (discovery cohort AUC = 0.836, validation cohort AUC = 0.840). Metabolomic analysis of feces, serum and urine revealed alterations in amino acid metabolism and disruptions in tetrahydrobiopterin (BH4) synthesis in the SRBARC, which affected serotonin (5-HT) and dopamine (DA) pathways. Four metabolites, including 5-methoxytryptophol, were identified as potentially metabolites associated with emotional states. These characteristic microorganisms and metabolic features were associated with neuroinflammation and immunity, and their interactions may play a key role in regulating emotional states in SRBARC. In summary, this study suggested a potential interplay among gut microbiota, neurotransmitter metabolism, and emotional states in the SRBARC, identifying potential key microbial signatures and metabolites that may provide a theoretical foundation for developing gut microbiotabased intervention strategies.