文献类型：期刊文献

英文题名：Schizochytrium limacinum valorizes volatile fatty acid-rich dark fermentative effluent for docosahexaenoic acid production

作者：Zhang, Yingqiu[1];Chen, Cheng[1];Wei, Kaixin[1];Sun, Liyun[1,2];Tan, Gaoyi[1];Zhang, Xu[1];Fan, Jianhua[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[3]Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832003, Peoples R China

年份：2025

卷号：434

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20252318568402);WOS:【SCI-EXPANDED(收录号:WOS:001509685700003)】；

基金：This work was sponsored by National Key Research and Development Project of China 2020YFA0907304, Shanghai Science and Technology Innovation Action Plan 24HC2820800, Natural Science Foundation of Shanghai 23ZR1415100 and 24ZR1490800, Ningbo "Innovation Yongjiang 2035" Key R & D Programme-International Sci-tech Cooperation Projects 2024H002, Funding Project of the State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.

语种：英文

外文关键词：Wastewater utilization; Schizochytrium limacinum SR21; Butyric acid; Biotransformation; Fatty acid; DHA

摘要：Dark fermentation wastewater, often rich in volatile fatty acids (VFAs), presents significant treatment challenges but also holds potential value. Oil-producing microorganisms can efficiently convert high VFAs concentrations into intracellular lipids. This study explores how VFAs utilization by Schizochytrium limacinum SR21 affects microbial fatty acid accumulation. It was found that S. limacinum has high VFAs biotransformation ability. The results show that butyric acid, as the sole carbon source, significantly enhances S. limacinum SR21 growth and docosahexaenoic acid (DHA) production, with utilization rate of 100 % and DHA yields of 2.0 g/L under optimal conditions. In actual wastewater applications, DHA production reached 1.5 g/L, with total phosphorus and chemical oxygen demand (COD) removal efficiencies of 41.2 % and 52.7 %, respectively. This approach not only addresses the treatment of dark fermentation wastewater but also enables polyunsaturated fatty acids (PUFAs) production from accumulated lipids, supporting both environmental and energy sustainability.