文献类型：期刊文献

中文题名：Safe antifungal lipopeptides derived from Bacillus marinus B-9987 against grey mold caused by Botrytis cinerea

英文题名：Safe antifungal lipopeptides derived from Bacillus marinus B-9987 against grey mold caused by Botrytis cinerea

作者：Gu Kang-bo[1];Zhang Dao-jing[1];Guan Cheng[1];Xu Jia-hui[1];Li Shu-lan[2];Shen Guo-min[1];Luo Yuan-chan[1];Li Yuan-guang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Zeyuan Marine Biotechnol Co Ltd, Shanghai 201203, Peoples R China

年份：2017

卷号：16

期号：9

起止页码：1999

中文期刊名：Journal of Integrative Agriculture

外文期刊名：JOURNAL OF INTEGRATIVE AGRICULTURE

收录：CSTPCD;;Scopus;WOS:【SCI-EXPANDED(收录号:WOS:000411047600012)】；CSCD:【CSCD2017_2018】；

基金：This work was financially supported by the Key Technologies Research and Development Program of China (2011BAE06B04-16). Moreover, the authors would like to thank the State Key Laboratory of Bioreactor Engineering of East China University of Science and Technology, Shanghai Zeyuan Marine Biotechnology Co. Ltd., Shanghai Branch of National Pesticide Research & Development South Center, Institute of Agricultural Environment & Resource of the Yunnan Academy of Agricultural Sciences (YAAS), and Zhejiang Research Institute of Chemical Industry, China, for their assistances in the technologies and instruments for scaled-up purification, scaled-up fermentation, pot tests, field experiments and systemic acute toxicity tests, respectively.

语种：英文

中文关键词：Bacillus marinus marine-derived lipopeptide field experiment acute toxicity test low-toxicity substance swarming motility Botrytis cinerea

外文关键词：Bacillus marinus; marine-derived lipopeptide; field experiment; acute toxicity test; low-toxicity substance; swarming motility; Botlytis cinerea

摘要：Agricultural application studies, including field experiments and acute toxicity tests, were conducted for lipopeptides secreted by marine-derived Bacillus marinus B-9987. Benefiting from commercially available scaled-up lipopeptide purification, the sample of impurities（isolated from target lipopeptides）, raw extracted sample（purity： 9.08%）, partially purified sample（purity： 20.86%）, and highly purified sample（purity： 87.51%） were prepared from B. marinus B-9987 fermentation broth, and used in lab-scale antagonism tests, field experiments, swarming motility tests, and acute toxicity tests. Operations and conditions in field experiments were consistent with the Pesticide-Guidelines for the Field Efficacy Trials（GB/T 17980.28-2000）, and acute toxicity tests were executed according to Toxicological Test Methods of Pesticides for Registration（GB 15670-1995）. In agar diffusion tests in vitro and pot tests in vivo, all lipopeptide samples with different purities significantly inhibited Botrytis cinerea; meanwhile the sample of impurities isolated from target lipopeptides were not effective against B. cinerea. Results of lab-scale tests showed that the target lipopeptides were effective substances against B. cinerea. Thus, partially purified and raw extracted samples were used in field experiments instead of the highly purified sample for cost saving. In the field experiments against rose grey mold, biological control efficacy of 500 mg L–1 lipopeptides reached 67.53%, slightly lower than 74.05% reached by the agrochemical pyrimethanil. However, pyrimethanil severely suppressed B. marinus B-9987, whereas the lipopeptides promoted swarming motility and biocontrol efficacy of Bacillus biomass. Lipopeptides at 87.51% purity were tested for systemic acute toxicity and confirmed as low-toxicity substances. In conclusion, low-toxicity lipopeptides were potential alternatives to agrochemicals, and they also performed good promotion when combined with homologous biological control microorganism. There were 2 breakthroughs in this research：（1） marine-derived bacterial lipopeptides inhibited grey mold caused by B. cinerea in field experiments; and（2） purified bacterial lipopeptides（sample purity： 〉87.51%） were determined to be low-toxicity substances by systemic acute toxicity tests, satisfying the strict requirement of pesticide registration in China（required purity： 〉85%）. This study provides support for using extracellularBacillus-derived lipopeptides commercially similar to Bacillus-based biological control agents.
Agricultural application studies, including field experiments and acute toxicity tests, were conducted for lipopeptides secreted by marine-derived Bacillus marinus B-9987. Benefiting from commercially available scaled-up lipopeptide purification, the sample of impurities (isolated from target lipopeptides), raw extracted sample (purity: 9.08%), partially purified sample (purity: 20.86%), and highly purified sample (purity: 87.51%) were prepared from B. marinus B-9987 fermentation broth, and used in lab-scale antagonism tests, field experiments, swarming motility tests, and acute toxicity tests. Operations and conditions in field experiments were consistent with the Pesticide-Guidelines for the Field Efficacy Trials (GB/T 17980.28-2000), and acute toxicity tests were executed according to Toxicological Test Methods of Pesticides for Registration (GB 15670-1995). In agar diffusion tests in vitro and pot tests in vivo, all lipopeptide samples with different purities significantly inhibited Botrytis cinerea; meanwhile the sample of impurities isolated from target lipopeptides were not effective against B. cinerea. Results of lab-scale tests showed that the target lipopeptides were effective substances against B. cinerea. Thus, partially purified and raw extracted samples were used in field experiments instead of the highly purified sample for cost saving. In the field experiments against rose grey mold, biological control efficacy of 500 mg L-1 lipopeptides reached 67.53%, slightly lower than 74.05% reached by the agrochemical pyrimBacillus-derived lipopeptides commercially similar to Bacillus-based biological control agents.ethanil. However, pyrimethanil severely suppressed B. marinus B-9987, whereas the lipopeptides promoted swarming motility and biocontrol efficacy of Bacillus biomass. Lipopeptides at 87.51% purity were tested for systemic acute toxicity and confirmed as low-toxicity substances. In conclusion, low-toxicity lipopeptides were potential alternatives to agrochemicals, and they also performed good promotion when combined with homologous biological control microorganism. There were 2 breakthroughs in this research: (1) marine-derived bacterial lipopeptides inhibited grey mold caused by B. cinerea in field experiments; and (2) purified bacterial lipopeptides (sample purity: >87.51%) were determined to be low-toxicity substances by systemic acute toxicity tests, satisfying the strict requirement of pesticide registration in China (required purity: >85%). This study provides support for using extracellular Bacillus-derived lipopeptides commercially similar to Bacillus-based biological control agents.