文献类型：期刊文献

中文题名：绿豆环氧水解酶催化对硝基苯乙烯氧化物的对映归一性水解

英文题名：Enantioconvergent Hydrolysis of p-Nitrostyrene Oxide Catalyzed by Mung Bean Epoxide Hydrolase

作者：鞠鑫[1];潘江[1];许建和[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2008

卷号：29

期号：8

起止页码：696

中文期刊名：催化学报

外文期刊名：Cuihua Xuebao / Chinese Journal of Catalysis

收录：CSTPCD;;EI(收录号：20084411672033);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(20576037;20672037)

语种：中文

中文关键词：绿豆;环氧水解酶;对硝基苯乙烯氧化物;对映归一性水解;(R)-对硝基苯乙二醇

外文关键词：mung bean ; epoxide hydrolase ; p-nitrostyrene oxide; enantioconvergent hydrolysis; （ R ）-p-nitro-phenylglycol

摘要：利用绿豆环氧水解酶催化(R,S)-对硝基苯乙烯氧化物水解,产物(R)-对硝基苯乙二醇的产率超过常规酶促拆分的极限产率(50%),表明发生了对映体会聚.采用绿豆粉剂和粗酶制剂考察了不同条件下的生物催化反应,结果表明,添加吐温-80作为分散剂对反应有促进作用.对该酶的固定化进行了初步研究,发现酶经硅藻土吸附固定化后稳定性显著增加.在克级规模制备实验中,产物的总产率达73.3%;产物经一步重结晶,得到了光学纯度大于99%的(R)-对硝基苯乙二醇.
Mung bean epoxide hydrolase was used for enzymatic preparation of （R）-p-nitrophenyl glycol by enantioconvergent hydrolysis of p-nitrostyrene oxide. Enzyme forms and reaction conditions were examined, and an efficient process and high product yield were obtained for this biocatalyst. Tween-80 was found to be an ideal dispersant. Various immobilized biocatalysts were prepared by different methods, and the biocatalyst adsorbed on diatomite powder showed better stability. Gram-scale preparation was performed, giving a yield of 73,3 % and an optical purity higher than 99 % ee,