文献类型：期刊文献

英文题名：Bioactivity-guided separation of antifungal compounds by preparative high-performance liquid chromatography

作者：Li, Zhidong[1];Dai, Zhuoshun[1];Jiang, Dasen[1];Dai, Yingping[1];Jin, Yu[1];Fu, Qing[1];Liang, Xinmiao[1,2]

机构：[1]East China Univ Sci & Technol, Sch Pharm, Minist Educ, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China

年份：2021

卷号：44

期号：12

起止页码：2382

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20211910341251);WOS:【SCI-EXPANDED(收录号:WOS:000649247300001)】；

基金：This work was supported by "National Science and Technology Major Project (2018ZX09735-002) ".

语种：英文

外文关键词：activity screening; antifungals; polyoxins; preparative high‐ performance liquid chromatography

摘要：Bioactivity-guided chromatographic methods are of great significance for the isolation of the active compounds in complex samples. In this study, four anti-fungal compounds were located by activity screening and successfully isolated from a microbial fermentation sample by preparative high-performance liquid chromatography. Separation performance of columns including C18, positively charged C18, negatively charged C18 and C8 were firstly investigated. And it showed a better capacity of mixed-mode stationary phases for retention and separation. Therefore, the positively charged C18 column was used to separate the sample into several fractions, among which the active one was identified by the antifungal test. And then the active fraction was enriched and separated again by successively using the negatively charged C18 and C8 columns to obtain four compounds, which were identified as polyoxins A, K, F and H. With activity verification, four polyoxins were found to have good inhibitory effects against the three fungal plant diseases including rice sheath blight, tomato grey mould disease, and apple spot leaf disease.