文献类型：期刊文献

英文题名：Enhancement of Emodin Production by Medium Optimization and KH2PO4 Supplementation in Submerged Fermentation of Marine-Derived Aspergillus favipes HN4-13

作者：Qiu, Xiaohan[1];Gong, Lizhi[1];Xin, Xiujuan[1];An, Faliang[1]

机构：[1]East China Univ Sci & Technol, Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2021

卷号：19

期号：8

外文期刊名：MARINE DRUGS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000690615500001)】；

基金：This work was funded by the National Key R&D Program of China (2018YFC1706206, 2019YFC0312504) and the National Natural Science Foundation of China (41876189, 81703388). This work was also supported by State Key Laboratory of Bioreactor Engineering and the Open Research Fund Program of Institute of Regulatory Science.

语种：英文

外文关键词：emodin; Aspergillus favipes HN4-13; KH2PO4; submerged fermentation

摘要：Emodin is a widely distributed anthraquinone derivative with a variety of biological activities, one that can be efficiently produced by marine-derived fungus Aspergillus favipes HN4-13. However, its relatively low fermentation yield limits further development and pharmaceutical research work. In this study, Plaekett-Burman design and central composite design were adopted to optimize the fermentation conditions of A. favipes HN4-13. Optimal fermentation conditions in a 250-mL Erlenmeyer flask with 50 mL of medium were 59.3 g/L soluble starch, 10 g/L yeast extract paste, 30 g/L seawater salt, 1.04 g/L KH2PO4, 0.05 g/L MgSO4 center dot 7H(2)O, 0.01 g/L FeSO4 center dot 7H(2)O, seed culture 24 h, pH 5, inoculum size 18%, culture temperature 32 degrees C, and shaking at 160 rpm/min for 7 days. The production of emodin could achieve 132.40 +/- 3.09 mg/L, with no significant difference from the predicted value (132.47 mg/L). Furthermore, KH2PO4 supplementation strategy was employed to regulate the mycelial morphology, upregulate the transcriptional level of biosynthesis gene cluster, and enhance emodin production (185.56 +/- 4.39 mg/L).