文献类型：期刊文献

英文题名：Consolidated Bioprocessing of Highly Concentrated Jerusalem Artichoke Tubers for Simultaneous Saccharification and Ethanol Fermentation

作者：Guo, Lihao[1];Zhang, Jian[1];Hu, Fengxian[1];Ryu, Dewey Dy[2];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Univ Calif Davis, Biochem Engn Program, Davis, CA 95616 USA

年份：2013

卷号：110

期号：10

起止页码：2606

外文期刊名：BIOTECHNOLOGY AND BIOENGINEERING

收录：;EI(收录号：20142217762041);WOS:【SCI-EXPANDED(收录号:WOS:000327725400006)】；

基金：Contract grant sponsor: National Basic Research Program of ChinaContract grant number: 2011CB707406Contract grant sponsor: National High-Tech Program of ChinaContract grant number: 2012AA022301Contract grant sponsor: China Postdoctoral Science FoundationContract grant number: 2011M500742/2012T50380Contract grant sponsor: Fundamental Research Funds for the Central Universities of ChinaContract grant number: WF0913005/1114054/1214025Contract grant sponsor: Shanghai Leading Academic Discipline ProjectContract grant number: B505

语种：英文

外文关键词：consolidated bioprocessing (CBP); Jerusalem artichoke tuber (Jat); Saccharomyces cerevisiae DQ1; inulin; inulinase; high titer ethanol

摘要：Consolidated bioprocessing (CBP) of Jerusalem artichoke tuber (Jat) for ethanol production is one of the most promising options for an alternate biofuel technology development. The technical barriers include the weak saccharolytic enzyme (inulinase) activity of the fermentation strain, and the well mixing of the high viscous fermentation slurry at the highly concentrated Jat loading. In this study, Saccharomyces cerevisiae DQ1 was found to produce relatively large amount of inulinase for hydrolysis of inulin in Jat, and the helical ribbon stirring bioreactor used provided well mixing performance under the high Jat loading. Even a highly concentrated Jat loading up to 35% (w/w) in the helical ribbon bioreactor for CBP was allowed. The results obtained from this study have demonstrated a feasibility of developing a CBP process technology in the helical ribbon bioreactor for ethanol production at a high yield 128.7g/L and the theoretical yield 73.5%, respectively. This level of ethanol yield from Jat is relatively higher than others reported so far. The results of this study could provide a practical CBP process technology in the helical ribbon bioreactor for economically sustainable alternate biofuel production using highly concentrated inulin containing biomass feedstock such as Jat, at least 35%. Biotechnol. Bioeng. 2013;110: 2606-2615. (c) 2013 Wiley Periodicals, Inc.