文献类型：期刊文献

英文题名：Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coating

作者：Ren, Yuhong[1];Rivera, Jose G.[2,3];He, Lihong[2,3];Kulkarni, Harsha[2,3];Lee, Dong-Keun[2,3];Messersmith, Phillip B.[2,3,4,5,6,7]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA;[3]Northwestern Univ, Chem Life Proc Inst, Evanston, IL 60208 USA;[4]Northwestern Univ, Chem & Biol Engn Dept, Evanston, IL 60208 USA;[5]Northwestern Univ, Mat Sci & Engn Dept, Evanston, IL 60208 USA;[6]Northwestern Univ, Inst Bionanotechnol Med, Evanston, IL 60208 USA;[7]Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Evanston, IL 60208 USA

年份：2011

卷号：11

外文期刊名：BMC BIOTECHNOLOGY

收录：;EI(收录号：20112614094932);WOS:【SCI-EXPANDED(收录号:WOS:000292032900001)】；

基金：This research was partially supported by grant R37 DE 014193 from the National Institutes of Health.

语种：英文

外文关键词：Nanomagnetics - Cost effectiveness - Biomimetics - Enzyme immobilization - Iron oxides - Metal nanoparticles

摘要：Background: Immobilization of lipase on appropriate solid supports is one way to improve their stability and activity, and can be reused for large scale applications. A sample, cost-effective and high loading capacity method is still challenging. Results: A facile method of lipase immobilization was developed in this study, by the use of polydopamine coated magnetic nanoparticles (PD-MNPs). Under optimal conditions, 73.9% of the available lipase was immobilized on PD-MNPs, yielding a lipase loading capacity as high as 429 mg/g. Enzyme assays revealed that lipase immobilized on PD-MNPs displayed enhanced pH and thermal stability compared to free lipase. Furthermore, lipase immobilized on PD-MNPs was easily isolated from the reaction medium by magnetic separation and retained more than 70% of initial activity after 21 repeated cycles of enzyme reaction followed by magnetic separation. Conclusions: Immobilization of enzyme onto magnetic iron oxide nanoparticles via poly-dopamine film is economical, facile and efficient.