文献类型：期刊文献

中文题名：常压常温等离子体(ARTP)诱变选育顶头孢霉高产菌

英文题名：High throughput screening of high-yield cephalosporin C producing strain by mutagenesis using atmospheric and room temperature plasma(ARTP)

作者：李英英[1];杭海峰[1];庄英萍[1];储炬[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2017

卷号：42

期号：11

起止页码：945

中文期刊名：中国抗生素杂志

外文期刊名：Chinese Journal of Antibiotics

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD_E2017_2018】；

基金：科技部国家重大科学仪器设备开发专项(No.2012YQ15008706)

语种：中文

中文关键词：头孢菌素C;ARTP;菌株选育;发酵

外文关键词：Cephalosporin C; ARTP; Fermentation; Strain screening

摘要：目的获得高产的工业微生物产生菌。方法采用ARTP诱变的方式来获得头孢菌素C的高产突变株。通过对突变菌株采用混合培养筛选与高通量生物检测相结合的高效筛选方法。结果最终选育获得高产菌株G6。与出发菌株W-6相比,G6在摇瓶中产头孢菌素C的能力提高了19.75%。结论 ARTP诱变方法是一种高效的头孢菌素C高产菌株选育方法。其正突变几率高,可有效提高菌株的头孢菌素C产量。
Objective To improve microbial strains for the high-production of industrial products.Methods In this paper,in order to obtain a cephalosporin C （CPC） high-yield stain,atmospheric and room temperature plasma （ARTP） was employed to generatethe mutants.In this study,all the mutants were given the equal screening chance by a mixture culture method combined with a high-throughput screening bioassay.Results The high-yield mixture strain G6 was successfully screened out and the CPC titer was nearly 19.75%,higher than that of the parental strain W-6 in the shake flask.Conclusion This work provides an appropriate strategy for obtaining high CPC-yield strains by using ARTP random mutation methods with an efficient screening assay.The ARTP random mutation method has a high positive mutation rate and the yield of cephalosporin C is significantly increased.