文献类型：期刊文献

英文题名：Ratiometric Detection of β-Amyloid and Discrimination from Lectins by a Supramolecular AIE Glyconanoparticle

作者：Zhang, Jun-Da[1,2];Mei, Ju[1,2];Hu, Xi-Le[1,2];He, Xiao-Peng[1,2];Tian, He[1,2]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Inst Fine Chem, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：12

期号：47

起止页码：6562

外文期刊名：SMALL

收录：;EI(收录号：20163102651548);WOS:【SCI-EXPANDED(收录号:WOS:000393220700012)】；

基金：J.-D.Z. and J.M. contributed equally to this work. The authors thank the 973 Project (Grant No. 2013CB733700), the National Natural Science Foundation of China (Grant No. 21572058), the Shanghai Rising-Star Program (Grant No. 16QA1401400) (Dr. X.-P. He) and the Shanghai Sailing Program (Grant No. 16YF1402200) (Dr. J. Mei) for generous financial support.

语种：英文

外文关键词：Energy transfer - Supramolecular chemistry - Forster resonance energy transfer - Neurodegenerative diseases - Glycoproteins - Proteins

摘要：While the development of AIE (aggregation-induced-emission) based fluorimetric probes for biological applications has been an active research area, probes with a ratiometric signal for biomolecular recognition have been rare. Here, a ratiometric AIE glyconanoparticle formed by the supramolecular assembly between a silole-based AIEgen and fluorescent glycoprobes for the detection of amyloid beta (A beta) peptides and fibrils, which are a signature of neurological disorders such as the Alzheimer's disease, is shown. Complexation of glycoprobes with the AIEgen produces an intensive fluorescence emission of the former because of a Forster resonance energy transfer between the two molecules. Subsequently, the presence of A beta dissembles the particle, producing a fluorescence emission of the AIEgen. Interestingly, the addition of lectins that selectively recognize the glycoprobes results in a different ratiometric response of the particle, thereby enabling a discrimination from A beta detection. This research offers insight into the simple construction of multifunctional ratiometric probes based on the supramolecular hybridization of a wide variety of AIEgens with fluorescent molecular probes.