文献类型：期刊文献

英文题名：Enhancement of lipid productivity with a novel two-stage heterotrophic fed-batch culture of Chlorella protothecoides and a trial of CO2 recycling by coupling with autotrophic process

作者：Wang, Tao[1];Tian, Xiwei[1];Liu, Tingling[1];Wang, Zejian[1];Guan, Wenyan[1];Guo, Meijin[1,2];Chu, Ju[1,2];Zhuang, Yingping[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, POB 329,130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：95

起止页码：235

外文期刊名：BIOMASS & BIOENERGY

收录：;EI(收录号：20164703044037);WOS:【SCI-EXPANDED(收录号:WOS:000389288900027)】；

基金：This work was supported by the National Basic Research Program of China (973 Project) (Grant No. 2013CB733600 and 2012CB72100X), National High Technology Research and Development Program (2015AA021005), and Major Science and Technology Program for Water Pollution Control and Treatment, 2014ZX07104-006. Many thanks to Cees Haringa for his careful polish on English language.

语种：英文

外文关键词：Microalgae; Chlorella protothecoides; Lipid; Two-stage oxygen supply; Fed-batch culture; Dual-mode culture

摘要：In this study, the influences of oxygen supply and nitrogen starvation on cell growth and lipid production by heterotrophic culture of Chlorella protothecoides were investigated in a 5-L stirred-tank bioreactor. The results demonstrated that both a low oxygen supply and nitrogen starvation are favorable for lipid accumulation, but limit cell growth and may therefore reduce the overall lipid production. Hence, a novel two-stage strategy for oxygen supply and nitrogen starvation was proposed in a batch culture, increasing the lipid content and yield on glucose by a factor 2.14 and 2.03 compared to the one-stage batch culture with high oxygen supply and sufficient nitrogen availability, respectively. Applying the two-stage process in a fed-batch culture further increased the lipid productivity to 175.2 mg/(L.h), giving a lipid yield of 195.6 mg/g. Furthermore, a dual-mode culture, coupling a heterotrophic process with autotrophic process, was implemented to allow for CO2 recycling. This improved the lipid productivity and carbon utilization efficiency, further contributing towards cost-effective and environment-friendly algal lipid production. (C) 2016 Elsevier Ltd. All rights reserved.