文献类型：期刊文献

英文题名：A genomic search approach to identify carbonyl reductases in Gluconobacter oxydans for enantioselective reduction of ketones

作者：Chen, Rong[1,2];Liu, Xu[1];Lin, Jinping[1];Wei, Dongzhi[1]

机构：[1]E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Hangzhou Normal Univ, Ctr Biomed & Hlth, Div Bas Med, Hangzhou, Zhejiang, Peoples R China

年份：2014

卷号：78

期号：8

起止页码：1350

外文期刊名：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY

收录：;EI(收录号：20152901038072);WOS:【SCI-EXPANDED(收录号:WOS:000340722500009)】；

基金：This work was financially supported by the National Key Basic Research Development Program of China ["973" Program, No. 2012CB721003], the Natural Science Foundation of China [No. 21002029/B020706 and 21276084/B060804] and National Major Science and Technology Projects of China [No. 2012ZX09304009], Natural Science Foundation of Zhejiang Province LY13C050004, Science and Technology Development Foundation of Hangzhou [20101131N05], Technology Research and Development Program for Institute of Hangzhou [20130432B05].

语种：英文

外文关键词：carbonyl reductase; enantioselective reduction; chiral alcohols

摘要：The versatile carbonyl reductases from Gluconobacter oxydans in the enantioselective reduction of ketones to the corresponding alcohols were exploited by genome search approach. All purified enzymes showed activities toward the tested ketoesters with different activities. In the reduction of 4-phenyl-2-butanone with in situ NAD(P)H regeneration system, (S)-alcohol was obtained with an e.e. of up to 100% catalyzed by Gox0644. Under the same experimental condition, all enzymes catalyzed ethyl 4-chloroacetoacetate to give chiral products with an excellent e.e. of up to 99%, except Gox0644. Gox2036 had a strict requirement for NADH as the cofactor and showed excellent enantiospecificity in the synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate. For the reduction of ethyl 2-oxo-4-phenylbutyrate, excellent e.e. (>99%) and high conversion (93.1%) were obtained by Gox0525, whereas the other enzymes showed relatively lower e.e. and conversions. Among them, Gox2036 and Gox0525 showed potentials in the synthesis of chiral alcohols as useful biocatalysts.