文献类型：期刊文献

中文题名：钙离子对鼠角质细胞生长和分化的影响

英文题名：Calcium-regulated Growth and Differentiation of the Mouse Epidermal Keratinocytes

作者：于海强[1];周燕[1];华平[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2002

卷号：18

期号：5

起止页码：626

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：MEDLINE(收录号：12561213);CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家高技术研究发展计划课题 (No .2 0 0 1AA2 10 6 41);国家重点基础研究发展规划"组织工程的基本科学问题"项目(No .G19990 5 430 9)的资助~~

语种：中文

中文关键词：钙离子;鼠;角质细胞生长;细胞培养;细胞分化;细胞老化

外文关键词：keratinocytes, cell culture, cell differentiation, cell senescence

摘要：利用无血清培养基培养角质细胞 ,研究了Ca2 +对鼠角质细胞生长和分化的影响。实验结果表明 ,培养基中钙离子最佳浓度为 0 2mmol L。在此浓度下 ,细胞克隆形成率达到 10 8% ,细胞的贴壁率达到 2 8 7% ,细胞的分化比例和老化比例分别为 5 4%和 2 6 3% ;当Ca2 +浓度达到 0 6mmol L以上时 。
The effect of the calcium ion (Ca 2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8 %, 30.8 %, 5.1 %, and 26.8 %, respectively. However, the Ca 2+ concentrations in the medium above 0.6mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.