文献类型：期刊文献

英文题名：Anti-metastatic effect and mechanisms of Wenshen Zhuanggu Formula in human breast cancer cells

作者：Han, Xiang-hui[1];Wang, Chun-li[2];Xie, Yan[3];Ma, Jiang[4];Zhang, Xiao-hui[2];Hu, Qian-wen[1];Lin, Ge[4];Liu, Sheng[1];Lu, Jin-gen[1]

机构：[1]Shanghai Univ Tradit Chinese Med, Inst Chinese Tradit Surg, Longhua Hosp Affiliated, Shanghai 200032, Peoples R China;[2]E China Univ Sci & Technol, Engn Res Ctr Modernizat Tradit Chinese Med, Shanghai 200237, Peoples R China;[3]Shanghai Univ Tradit Chinese Med, Res Ctr Hlth & Nutr, Shanghai 201203, Peoples R China;[4]Chinese Univ Hong Kong, Sch Biomed Sci, Fac Med, Hong Kong, Hong Kong, Peoples R China

年份：2015

卷号：162

起止页码：39

外文期刊名：JOURNAL OF ETHNOPHARMACOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000351323400006)】；

基金：The authors are grateful for the financial support from National Natural Science Foundation of China (Nos. 81102598 and 81173269) and Program for Longhua Medical Research Team (No. LYTD-17).

语种：英文

外文关键词：Wenshen Zhuanggu Formula; Traditional Chinese medicine; Mesenchymal stem cells; Breast cancer; Metastases

摘要：Ethnopharmacological relevance: Wenshen Zhuanggu Formula (WSZG), a traditional Chinese medicine (TCM) empirical prescription, has been used to treat the patients with breast cancer bone metastasis as an adjuvant in clinical practice. To explore the anti-metastatic activity and potential mechanisms of WSZG-containing serum (WSZG-CS) on highly bone-metastatic human breast cancer MDA-MB-231BO cells. Materials and methods: MDA-MB-231BO cells were cultured alone or co-cultured with bone marrow-derived mesenchymal stem cells (BMSCs). Invasion assays were carried out in Matrigel-coated Transwell chambers. CC chemokine 5 (CCL5) and interleukin (IL)-17B secretion levels were detected by ELISA. CCR5 and IL-17BR protein expression levels were determined by immunocytochemistry and Western blot analysis. Results: Compared with control serum, WSZG-CS significantly inhibited BMSC induced MDA-MB-231B0 breast cancer cell invasion, reduced CCL5 and IL-17B levels in co-culture supernatants, and down-regulated CCR5 and IL-17BR protein expression in breast cancer cells co-cultured with BMSCs. Conclusions: WSZG-CS exerts an anti-metastatic activity against MDA-MB-231B0 breast cancer cells, due to its ability to mitigate the interaction between BMSCs and breast cancer cells mediated via the CCL5/CCR5 and IL-17B/IL-17BR signaling pathways. (C) 2014 Elsevier Ireland Ltd. All rights reserved.