文献类型：期刊文献

中文题名：用大肠杆菌游离细胞酶法合成核苷药物

英文题名：Characteristics in Biotransformation Process with Free E.coli Cells

作者：周长林[1];邱蔚然[1];周利[1];俞俊棠[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室

年份：1997

卷号：23

期号：5

起止页码：535

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家"九五"攻关基金

语种：中文

中文关键词：核苷药物;酶法;合成;核苷磷酸化酶;大肠杆菌

外文关键词：nucleoside pharmaceuticals;enzymatic synthesis;phosphorylase; E.coli

摘要：研究了采用大肠杆菌游离细胞核苷磷酸化酶合成核苷药物过程中反应平衡常数和底物转化率的计算方法及其变化规律，阐明了游离细胞内核苷磷酸化酶的活力与细胞的培养、菌体的预处理方式密切相关。实验结果表明，大肠杆菌湿菌体经４°Ｃ干燥处理２～３ｄ后，胞内核苷磷酸化酶不易失活，并能有效地降低胞内抑制物对酶反应的抑制作用。与湿菌体相比，干燥菌体用于酶反应时底物的转化率可提高３３．５％。
The calculation method of theoretical substrate conversion rate in the enzyma tic preparation of nucleoside pharmaceuticals catalyzed by phosphorylase from free E.coli cells was studied.In addition,It was shown that phosphorylase activity of free cells was closely related to their culture condition and pretreatment procedures.If wet E.coli cells were dried at 4°C 2～3 days,the stability of phosphorylase within cells was increased,the inhibition within cells reduced and the substrate conversion rate increased by 33.5%.