文献类型：期刊文献

英文题名：Rational Design of Near-Infrared Cyanine-Based Fluorescent Probes for Rapid In Vivo Sensing Cysteine

作者：Zhang, Hehe[1];Yan, Chenxu[1];Li, Hui[2];Shi, Lei[1];Wang, Ruofei[1];Guo, Zhiqian[1];Zhu, Wei-Hong[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai Key Lab Funct Mat Chem, Inst Fine Chem,Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Dept Radiol, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China

年份：2021

卷号：4

期号：3

起止页码：2001

外文期刊名：ACS APPLIED BIO MATERIALS

收录：EI(收录号：20201608476963);WOS:【ESCI(收录号:WOS:000630168200002)】；

基金：This work was supported by NSFC/China (21622602, 21788102, 21636002, 21878087, and 21908060), National Key Research and Development Program (2016YFA0200300), the Innovation Program of Shanghai Municipal Education Commission, Scientific Committee of Shanghai (15XD1501400), Shuguang Program (18SG27), and China Postdoctoral Science Foundation (Grant 2019M651417).

语种：英文

外文关键词：fluorescent probe; near infrared; rapid response; cyanine; in vivo imaging; cysteine

摘要：Cysteine (Cys) is well-known to be an important biothiol and related to many diseases. However, the in situ trapping of endogenous Cys is still handicapped by a lack of straightforward methods combined with long-wavelength emission and high-performance response. In this work, we described the rational design strategy of cyanine-based near-infrared (NIR) probes for the rapid detection of mitochondrial Cys in living cells and mice. We focus on how to improve the response rate via regulating the electron density of the recognition units in probes. The obtained three probes all displayed remarkable fluorescence enhancement at 780 nm. From screening the obtained probes, it was found that the probe Cy-S-diOMe with electron-donating recognition unit displayed the fastest response rate, the lowest detection limit, and the highest signal-to-noise ratio. More importantly, Cy-SdiOMe was successfully applied to monitor Cys in tumor-bearing mice (within merely 5 min). This paradigm by modulation of the response rate in the cyanine dyes provides a promising methodology for the design of high-performance cyanine-based NIR probes.