文献类型：期刊文献

英文题名：Continuous Production of Ursodeoxycholic Acid by Using Two Cascade Reactors with Co-immobilized Enzymes

作者：Zheng, Ming-Min[1];Chen, Fei-Fei[1];Li, Hao[1];Li, Chun-Xiu[1];Xu, Jian-He[1,2]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2018

卷号：19

期号：4

起止页码：347

外文期刊名：CHEMBIOCHEM

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000425511500007)】；

基金：This work was financially supported by the National Natural Science Foundation of China (no. 21536004), and Shanghai Commission of Science and Technology (no. 15JC1400403).

语种：英文

外文关键词：domino reactions; enzymes; immobilization; natural products; supported catalysts

摘要：Ursodeoxycholic acid (UDCA) is an effective drug for the treatment of hepatitis. In this study, 7-hydroxysteroid dehydrogenase (7-HSDH) and lactate dehydrogenase (LDH), as well as 7-hydroxysteroid dehydrogenase (7-HSDH) and glucose dehydrogenase (GDH), were co-immobilized onto an epoxy-functionalized resin (ES-103) to catalyze the synthesis of UDCA from chenodeoxycholic acid (CDCA). Through optimizing the immobilization pH, time, and loading ratio of enzymes to resin, the specific activities of immobilized LDH-7HSDH@ES-103 and 7HSDH-GDH@ES-103 were 43.2 and 25.8Ug(-1), respectively, which were 12- and 516-fold higher than that under the initial immobilization conditions. Continuous production of UDCA from CDCA was subsequently achieved by using immobilized LDH-7HSDH@ES-103 and 7HSDH-GDH@ES-103 in two serial packed-bed reactors. The yield of UDCA reached nearly 100% and lasted for at least 12h in the packed-bed reactors, which was superior to that of the batchwise reaction. This efficient continuous approach developed herein might provide a feasible route for large-scale biotransformation of CDCA into UDCA.