文献类型：期刊文献

中文题名：华夏^(TM)白金PCR扩增试剂盒的法医学应用评估

英文题名：Forensic Application of Huaxia^(TM) Platinum Kit

作者：王亚丽[1,2];盛翔[2,3];李敏[2,4];陈玉玲[2,5];林源[2];陈丽琴[1]

机构：[1]内蒙古医科大学法医学教研室,内蒙古呼和浩特010030;[2]司法部司法鉴定科学技术研究所上海市法医学重点实验室上海市司法鉴定专业技术服务平台,上海200063;[3]苏州大学医学部法医学系,江苏苏州215123;[4]温州医科大学基础医学院,浙江温州325000;[5]华东理工大学生物工程学院生物反应器工程国家重点实验室,上海200237

年份：2017

卷号：33

期号：2

起止页码：129

中文期刊名：法医学杂志

外文期刊名：Journal of Forensic Medicine

收录：CSTPCD;;Scopus;CSCD:【CSCD_E2017_2018】；PubMed;

基金：十三五国家重点研发计划项目(2016YFC0800703);上海市科委标准研制项目(16DZ0501600);上海市法医学重点实验室资助项目(17DZ2273200);上海市司法鉴定专业技术服务平台资助项目(16DZ2290900)

语种：中文

中文关键词：法医遗传学;短串联重复序列;多态现象,遗传;华夏TM白金PCR扩增试剂盒;汉族

外文关键词：forensic genetics; short tandem repeat; polymorphism, genetic; Huaxia Platinum kit; Han nationality

摘要：目的调查华夏^(TM)白金PCR扩增试剂盒所包含的23个常染色体STR基因座在中国汉族人群中的遗传多态性,评估其在法医遗传学中的应用价值。方法应用华夏^(TM)白金PCR扩增试剂盒对500名汉族无关健康个体进行分型检测,统计分析所含STR基因座的频率分布及群体遗传学参数,与目前国内外常用的7种商品化试剂盒在STR基因座个数、内标、荧光标记、Ladder中等位基因总数以及系统效能上进行比较。结果 23个常染色体STR基因座均符合Hardy-Weinberg平衡(P>0.05),个体识别率为0.791 5~0.986 2,多态信息含量为0.559 0~0.914 0。系统累积个体识别率高达1-4.1×10-28,三联体累积非父排除率为1-4.1×10^(-10),二联体累积非父排除率为1-8.4×10^(-7)。通过与7种试剂盒比较,华夏^(TM)白金PCR扩增试剂盒含有的等位基因分型标准品(Ladder)中的等位基因数最多。结论 23个常染色体STR基因座在汉族人群中有良好的遗传多态性,可用于法医遗传学亲权鉴定及个体识别。通过与其他常见的7种商业化STR检测试剂盒比较,华夏^(TM)白金PCR扩增试剂盒可进一步提供更为丰富的遗传信息。
Objective To investigate the genetic polymorphism of 23 autosomal STR loci of Huaxia Platinum kit in Chinese Han population, and to evaluate the forensic efficiency of Huaxia Platinum kit. Methods A total of 500 unrelated healthy individuals from Han population were genotyped with Huaxia Platinum kit. The frequency distribution and the parameter of population genetics of STR loci were analysed statistically. Huaxia Platinum kit was compared with other 7 commercial STR kits commonly seen at home and abroad in the number of STR loci, interior label, fluorescent mark, total number of alleles in Ladder and system effectiveness. Results All the 23 autosomal STR loci were consistent with Hardy- Weinberg equilibrium （P〉0.05）. The discrimination power was 0.791 5-0.986 2. The polymorphism infor-mation content （PIC） was 0.559 0-0.914 0. The combined discrimination power （CDP） was 1-4.1×10-28, while combined probability of paternity exclusion in trio （CPET） and in duo （CPED） were 1-4.1×1010 and 1-8.4×10-7 ,respectively. Compared with other 7 kits, Huaxia Platinum kit contained the most number of alleles within the Ladder. Conclusion All the 23 autosomal STR loci of Huaxia Platinum kit with highly polymorphic in Han population can be used for paternity testing and individual identification. Compared with other 7 kits, it appears that Huaxia Platinum kit can provide more genetic information.