文献类型：期刊文献

英文题名：One-step immobilization-purification of enzymes by carbohydrate-binding module family 56 tag fusion

作者：Qin, Zhen[1,2];Lin, Si[1];Qiu, Yongjun[1,2];Chen, Qiming[1,2];Zhang, Yin[3];Zhou, Jiachun[1,2];Zhao, Liming[1,2]

机构：[1]East China Univ Sci & Technol, R&D Ctr Separat & Extract Technol Fermentat Ind, State Key Lab Bioreactor Engn, Sch Biotechnol, Shanghai 200237, Peoples R China;[2]SCICBT, Shanghai 200237, Peoples R China;[3]Chengdu Univ, Key Lab Meat Proc Sichuan, Chengdu 610106, Sichuan, Peoples R China

年份：2019

卷号：299

外文期刊名：FOOD CHEMISTRY

收录：;EI(收录号：20192707141579);WOS:【SCI-EXPANDED(收录号:WOS:000477668100038)】；

基金：This work was financially supported by the National Natural Science Foundation of China (31701537), Shanghai Sailing Program (17YF1403500), Fundamental Research Funds for the Central Universities (222201814031, 22221818014), "Chen Guang" (17CG28) and "Shu Guang" (15SG28) project of Shanghai Municipal Education Commission and Shanghai Education Development Foundation.

语种：英文

外文关键词：Carbohydrate binding module; Immobilized enzyme; Packed-bed reactor; Fusion protein

摘要：Immobilization of enzymes is an essential strategy with outstanding prospects in biocatalytic processes. Nontoxic, inexpensive immobilized enzyme approach is especially important for food enzymes. We here demonstrate that a carbohydrate-binding module family 56 domain (CBM56-Tag) mediates the immobilization of fusion enzymes with the curdlan (beta-1,3-glucan) particle support, thereby enabling the one-step immobilization-purification of target enzymes. CBM56-Tag exhibits an immunoglobulin-like beta-sandwich fold, which can be adsorbed by curdlan via hydrogen bond-mediated binding. The maximum adsorption capacity of a fusion chitosanase (CBM56-GsCsn46A) on curdlan is 50.72 mg/g. The immobilized enzyme could be directly used in the packed-bed reactor. This immobilization strategy utilizes a natural polysaccharide without any treatment, avoiding the negative environmental effects. Moreover, the one step immobilization-purification simplifies the purification step, which reduces the use of chemicals. Our study provides a nontoxic and inexpensive immobilization strategy for the biocatalytic reaction in food industry.