文献类型：期刊文献

英文题名：Visible-light activatable coumarin-based phototriggers for fluorescence imaging with ultra-high photolysis efficiency

作者：Zhou, Renjie[1];Yang, Lipeng[2,3];Chen, Zhengda[3,4];Jiang, Li[2,3];Liu, Tuan[2];Wang, Zhiqiang[1];Huang, Xinyi[2];Lin, Qiuning[2];Gong, Xueqing[1];Yang, Yi[3,4];Zhu, Linyong[1,2,3]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Biomed Engn, 800 Dongchuan Rd, Shanghai 200240, Peoples R China;[3]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, 130 Mei Long Rd, Shanghai 200237, Peoples R China

年份：2023

卷号：25

期号：16

起止页码：11176

外文期刊名：PHYSICAL CHEMISTRY CHEMICAL PHYSICS

收录：;EI(收录号：20231613900237);WOS:【SCI-EXPANDED(收录号:WOS:000968305700001)】；

基金：This research was supported by the National Key Research and Development Program of China (2019YFA0904800 and 2019YFA011050), the National Natural Science Foundation of China (21937004, 22022506, 21877037, and 52103119), the Shanghai Pujiang Program (21PJ1405900), and the China Postdoctoral Science Foundation (2021M700087).

语种：英文

外文关键词：Efficiency - Fluorescence imaging - Scaffolds - Signal to noise ratio

摘要：Photoactivated fluorophores (PAFs) are powerful imaging tools for observing subcellular structures and tracking dynamic biological processes. However, photoremovable protecting groups (PPGs) widely used to construct PAFs suffer from the drawbacks of short-wavelength excitation and/or low photolysis efficiency. Herein, a class of coumarin-based PPGs with electron-rich thiophene derived substitutions at the C3-position of a coumarin scaffold were prepared. The modification not only leads to the redshift of the absorption band to the blue light region (400-500 nm), but also the increases of uncaging quantum yield (f(u)) as well as molar extinction coefficient (e(max)), thus enhancing the photolysis efficiency (f(u) x e(max)) up to 34.2 x 10(3) M-1 cm(-1). The exceptionally high photolysis efficiency enables efficient photolysis in blue light as weak as 2 mW cm(-2) or in blue light from a Luminol chemiluminescence system. Based on the excellent photolysis properties, the PAF constructed by the new PPG exhibits fast photoactivation and a low background signal, and the resulting fluorescence images display a signal-to-noise ratio greater than 780. It is anticipated that the superior photolysis performance makes the PPGs a novel platform for the construction of photo responsive systems in a variety of applications.