文献类型：期刊文献

英文题名：Computational fluid dynamics (CFD) modeling of Ilamycin E production in Streptomyces atratus SCSIO ZH16 submerged fermentation

作者：Zhou, Weiyan[1];Zheng, Gaofan[1];Wang, Jialuo[4];Xin, Xiujuan[1];Zhuang, Liwei[2];An, Faliang[1,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem Engn, Shanghai 200237, Peoples R China;[3]Marine Biomed Sci & Technol Innovat Platform Lin G, 4,Lane 218,Haiji Sixth Rd, Shanghai 201306, Peoples R China;[4]East China Univ Sci & Technol, Sch Math, Shanghai 200237, Peoples R China

年份：2027

卷号：338

外文期刊名：CHEMICAL ENGINEERING SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001835693600001)】；

基金：This work was financially supported by the National Key R&D Program of China (Nos. 2023YFA0914102, 2019YFC0312504), National Natural Science Foundation (81803381). This work was also supported by the Open Research Fund Program of State Key Laboratory of Bioreactor Engineering and Shanghai Collaborative Innovation Center for Biomanufacturing Technology.We thank Professor Jianhua Ju and Professor Junying Ma from South China Sea Institute of Oceanology for kindly providing us with the strain S. atratus SCSIO ZH16. We also extend our thanks to Dr. Ruida Wang and Dr. Ke Ma from East China University of Science and Technology for their valuable instruction in molecular experimental techniques.

语种：英文

外文关键词：Computational fluid dynamics; Bioprocess optimization; Viscosity; S.atratus SCSIO ZH16

摘要：A computational fluid dynamics (CFD) model was developed and validated against experiments for a laboratoryscale 5-L bioreactor. Numerical simulation was performed to describe the bioreaction of Streptomyces atratus SCSIO ZH16 fermentation for ilamycin E production with the dynamic changes in viscosity of the fermentation broth due to biomass growth and decay. This model can account for the two-way coupling between the fermentation environment and medium, which enabled the analysis of the relationship between the broth viscosity, flow field, mass transfer, and macroscopic fermentation performance. This work represents the first integration of Streptomyces fermentation with CFD, enabling the simulation of flow field and mass transfer under varying stirring speed, aeration rate, and viscosity during Streptomyces fermentation. A suggested favorable range of fermentation broth viscosity (10-30 mPa s) was identified for ilamycin E production by S. atratus SCSIO ZH16 fermentation. Furthermore, the addition of sorbitol was used to adjust the viscosity of the fermentation broth in the later stages of fermentation. Experimental evidence, including elevated OUR/CER and up-regulated respiratory-chain gene transcription, suggested that the yield improvement was primarily associated with enhanced oxygen transfer resulting from reduced broth viscosity. This research offers a practical strategy for the process intensification and industrial scale-up for such bioreactors.