文献类型：期刊文献

英文题名：Physicochemical investigations on the interactions between Gemini/single-chain cationic surfactants and bovine serum albumin

作者：Yin, Tianxiang[1];Qin, Miao[1];Shen, Weiguo[1,2]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Lanzhou Univ, Dept Chem, Lanzhou 730000, Gansu, Peoples R China

年份：2014

卷号：461

起止页码：22

外文期刊名：COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS

收录：;EI(收录号：20144500157501);WOS:【SCI-EXPANDED(收录号:WOS:000343351400004)】；

基金：This work was supported by the National Natural Science Foundation of China (Projects 21173080, 21373085, and 21303055).

语种：英文

外文关键词：Bovine serum albumin; Gemini surfactant; Spacer; Fluorescence; Circular dichroism

摘要：The interactions of Gemini surfactants 1-dodecyl-N,N'-[(1,4-dioxo-1,4-butanediyl)bis(oxy-2,1-ethanediyl)]bis[N,N'-dimethylammonium]dibromide (12-su-12) and 1-dodecyl-N,N'-[((2E)-1,4-dioxo-2-butene-1,4-diyl)bis(oxy-2,1-ethanediyl)]bis[N,N'-dimethylammonium]dibromide (12-fo-12) and the corresponding single-chain surfactant 1-dodecyl-N-[2-(acetyloxy)ethyl]-N,N-dimethyl ammonium bromide (DTAAB) with bovine serum albumin (BSA) were investigated by conductometry, fluorimetry, UV-vis and CD spectrometry, and dynamic light scattering. It was found that the bindings of all three surfactants to BSA were cooperatively driven by electrostatic and hydrophobic interactions. The Gemini surfactants, carrying more charges and hydrophobic tails, showed stronger interactions with BSA than the single-chain surfactant. The presence of the double bond in the spacer of 12-fo-12 enhanced the interaction between the surfactant and BSA and also significantly varied the binding sites as compared to 12-su-12 and DTAAB, which indicated an important role of the property of the spacer in the interaction between the surfactant and BSA. (C) 2014 Elsevier B.V. All rights reserved.