文献类型：期刊文献

英文题名：A Novel GH Family 20 β-N-acetylhexosaminidase With Both Chitosanase and Chitinase Activity From Aspergillus oryzae

作者：Qu, Tianle[1];Zhang, Chunyue[1];Qin, Zhen[2];Fan, Liqiang[1];Jiang, Lihua[1];Zhao, Liming[1,3]

机构：[1]East China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, R&D Ctr Separat & Extract Technol Fermentat Ind, Shanghai, Peoples R China;[2]Shanghai Univ, Sch Life Sci, Shanghai 3, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol SC, Shanghai, Peoples R China

年份：2021

卷号：8

外文期刊名：FRONTIERS IN MOLECULAR BIOSCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000656943600001)】；

基金：This work was financially supported by the National Key R&D Program of China (2019YFD0901805), the 111 Project (B18022) and the Fundamental Research Funds for the Central Universities (22221818014), and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering, ECUST (ZDXM 2019).

语种：英文

外文关键词：beta-N-acetylhexosaminidase; aminooligosaccharide; glycoside hydrolase family 20; chitosan; chitin

摘要：Aminooligosaccharides possess various biological activities and can exploit wide applications in food, pharmaceutical and cosmetic industries. Commercial aminooligosaccharides are often prepared by the hydrolysis of chitin and chitosan. In this study, a novel GH family 20 alpha-N-acetylhexosaminidases gene named AoNagase was cloned from Aspergillus oryzae and expressed in Pichia pastoris. The purified AoNagase had maximal activity at pH 5.5 and 65 degrees C. It exhibited good pH stability in the range of pH 6.0-7.5 and at temperatures below 50 degrees C. AoNagase was capable of hydrolyzing not only colloidal chitosan (508.26 U/mg) but also chitin (29.78 U/mg). The kinetic parameters (K-m and V-max) of AoNagase were 1.51 mM, 1106.02 U/mg for chitosan and 0.41 mM, 40.31 U/mg for colloidal chitin. To our knowledge, AoNagase is the first GH family 20 beta-N-acetylhexosaminidase capable of hydrolyzing both chitosan and chitin. AoNagase is an endo-type beta-N-acetylhexosaminidases and can potentially be used for the manufacturing of aminooligosaccharides.