文献类型：期刊文献

中文题名：硼基亲和层析介质的合成及其应用研究 I.基质的合成及孔结构

英文题名：Synthesis of the Media for the Boronate Affinity Choromatographic and Their ApplicationsI. Studied on the Synthesis and Structure of the Matrix

作者：宁方红[1,2];刘坐镇[1,2];诸蓓艳[1,2];刘勤凤[1,2];邬行彦[1,2];王彬芳[1,2]

机构：[1]华东理工大学生物反应器工程国家重点实验室;[2]华东理工大学高分子材料系

年份：1998

卷号：24

期号：5

起止页码：520

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金

语种：中文

中文关键词：大孔树脂;GMA;硼基;亲和层析

外文关键词：glycidyl methacrylate; ethylene dimethacrylate; m;aminophonyl boronic acid; covalant affinity chromatography

摘要：以甲基丙烯酸缩水甘油酯和二甲基丙烯酸乙二醇酯为原料，合成了大孔树脂，并研究了交联度、混合致孔剂用量和配比对其孔结构的影响。该树脂与间氨基苯硼酸偶联，制得硼基－聚甲基丙烯酸缩水甘油酯共价亲和层析介质，介质的硼基偶联量可达１．０３６ｍｍｏｌ／ｇ。
The macroreticular resin was preparted by the copolymerization of glycidyl methacrylate (GMA) with ethylene dimethacrylate(EDMA) and its pore structure was determined. The effects of the ammount of crosslinking degree and the amount as well as proportion of the mixed diluent on the pore structure were also investigated. The matrix of boric acid substituted PGMA covalant affinity chromatographic were prepared using the PGMA copled with m aminophonyl boronic acid and the capacity of the banic acid of the matrix was up to 1.036mmol/g.