文献类型：期刊文献

英文题名：The triggered antioxidant response and corresponding metabolomics expression of Caenorhabditis elegans for chronic exposure to moxifloxacin and trace copper

作者：Liu, Lili[1];Liu, Yuxia[1];Tang, Mingqi[1];Zhu, Manman[1];Wang, Fangfang[1];Lin, Kuangfei[1]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, Key Lab Environm Risk Assessment & Control Chem Pr, Minist Ecol & Environm, Shanghai 200237, Peoples R China

年份：2026

卷号：13

期号：1

外文期刊名：BIORESOURCES AND BIOPROCESSING

收录：;EI(收录号：20261320340577);WOS:【SCI-EXPANDED(收录号:WOS:001717393200003)】；

基金：This work was supported by the National Key Research and Development Program of China (2018YFC1901000) and National Natural Science Foundation of China (42577467).

语种：英文

外文关键词：Caenorhabditis elegans; Moxifloxacin; Copper; Antioxidant defenses; Metabolomics

摘要：As the terminal management for evaluating the engineering effectiveness of antibiotics production and utilization, the toxic effects of moxifloxacin (MOX) and trace concentration of Cu2+ (MOX-Cu) on Caenorhabditis elegans (C. elegans) were investigated at physiological, biochemical, and molecular level. Although the stimulate effects were observed after prolonged exposure (72 h) to MOX (0.2-2.0 mg/L), the expressions of HSPs, ace genes, and daf-16 were inhibited, indicating its adverse impact on cellular health, locomotion behaviors, and antioxidant defense of C. elegans. Similarly, the down-regulation of oxidative stress (sod-1 and daf-16) and cell damage (HSPs) related genes and the up-regulation of apoptosis-related genes (cep-1 and ape-1) indicated the oxidative stress and genotoxicity after prolonged exposure to MOX-Cu. For the chronic exposure (10 days) to MOX, the level of ROS was reduced due to the increased expressions of daf-16, sod-3, and hsp-16, accompanied with and the down-regulation of cep-1. Meanwhile, at the exposure to MOX-Cu, the levels of ROS and lipofuscin were decreased due to the up-regulation of sod-1 and daf-16, and the antioxidant defense was promoted and confirmed by the increase of amino acids and their related metabolic pathways. These results can provide a theoretical basis for the toxicity evaluation of typical antibiotics (MOX) that co-existing with trace heavy metals in natural environment media and bioresources processes.