文献类型：期刊文献

英文题名：Combining metabolic engineering and adaptive evolution to enhance the production of dihydroxyacetone from glycerol by Gluconobacter oxydans in a low-cost way

作者：Lu, Leifang[1]; Wei, Liujing[1]; Zhu, Kun[1]; Wei, Dongzhi[1]; Hua, Qiang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

年份：2012

卷号：117

起止页码：317

外文期刊名：Bioresource Technology

收录：EI(收录号：20122515128990)

语种：英文

外文关键词：Cost engineering - Metabolic engineering - Gene expression - Glycerol - Cultivation - Carbon

摘要：Gluconobacter oxydans can rapidly and effectively transform glycerol to dihydroxyacetone (DHA) by membrane-bound quinoprotein sorbitol dehydrogenase (mSLDH). Two mutant strains of GDHE Δ. adh pBBR-P. tufBsldAB and GDHE Δ adh pBBR- sldAB derived from the GDHE strain were constructed for the enhancement of DHA production. Growth performances of both strains were largely improved after adaptively growing in the medium with glucose as the sole carbon source. The resulting GAT and GAN strains exhibited better catalytic property than the GDHE strain in the presence of a high concentration of glycerol. All strains of GDHE, GAT and GAN cultivated on glucose showed enhanced catalytic capacity than those grown on sorbitol, indicating a favorable prospect of using glucose as carbon source to reduce the cost in industrial production. It was also the first time to reveal that the expression level of the sldAB gene in glucose-growing strains were higher than that of the strains cultivated on sorbitol. ? 2012 Elsevier Ltd.